4-Aminoantipyrine → Uric Acid

HRID 5761

反応式

HRID 5761 の反応式
CC1N(C)[N:6](C2C=CC=CC=2)[C:4](=[O:5])C=1N.C1C(=O)NC(=O)N(C2OC(COP(OC3C(O)C([N:41]4[C:47](=[O:48])[NH:46][C:44](=[O:45])[CH:43]=[CH:42]4)OC3CO)(O)=O)C(O)C2O)C=1.[NH3:53]>P([O-])([O-])([O-])=O>[NH:46]1[C:44](=[O:45])[C:43]2[NH:6][C:4](=[O:5])[NH:53][C:42]=2[NH:41][C:47]1=[O:48]

参加物

4

反応物 2

HCID 21514-AminoantipyrineHCID 14228836Urate Oxidase

生成物 1

HCID 1175Uric Acid

試薬・触媒・溶媒 1

HCID 1061Phosphate溶媒

反応条件

温度
37 °C

実験手順

To each of the mixtures was added a reagent prepared by dissolving 1 μmol 4-aminoantipyrine and 2 units of uricase in 1.5 ml of 0.1M phosphate buffer (pH 6.5). After the resulting mixtures were maintained in a thermostat at 37° C. for about 5 minutes, the absorbance was measured at a wavelength of 555 nm with a spectrophotometer (Model 228, manufactured by Hitachi, Ltd.) using purified water as a control. The results obtained are shown in Table 3.

後処理

  1. additionTo each of the mixtures was added a reagent
  2. customprepared
  3. customusing purified water as a control
  4. customThe results obtained

出典と根拠

ORDレコード
ord-f252bc5bba1b49918808b53ec81978dd
出典データセット
uspto-grants-1993_09
特許番号
US05246836

関連ノート

読み込み中…