(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) → HCID 21584101、化合物
反応式
[CH3:1][C:2]1[N:6]=[C:5]([N+:7]2[N:11]([C:12]3[CH:17]=[CH:16][CH:15]=[CH:14][CH:13]=3)[N:10]=[C:9]([C:18]3[CH:23]=[CH:22][CH:21]=[CH:20][CH:19]=3)[N:8]=2)[S:4][C:3]=1[CH3:24].[Br-:25]>C(O)(C)C>[Br-:25].[CH3:1][C:2]1[N:6]=[C:5]([N:7]2[N:8]=[C:9]([C:18]3[CH:23]=[CH:22][CH:21]=[CH:20][CH:19]=3)[NH2+:10][N:11]2[C:12]2[CH:17]=[CH:16][CH:15]=[CH:14][CH:13]=2)[S:4][C:3]=1[CH3:24]参加物
3
実験手順
Ten μl of thiazolyl blue tetrazolium bromide solution (5 mg/ml in PH 7.4 PBS) was added to each well containing 100 μl of medium and cells, followed by incubation at 37° C. for 4 hours in the dark. Isopropanol (100 μl, 0.04 N HCl) was added to dissolve blue dye. After dissolved completely, absorbance was measured with plate-reader (Synergy 2, BioTek Instrument) at test wavelength 570 nm and reference wavelength 670 nm. Cell viability was determined by absorbance at 570 nm and reported as ratio to control cells (without OGD).
後処理
- additioncontaining 100 μl of medium and cells
- dissolution