sodium (4R)-4-[(3R,5S,7R,8R,9R,10S,12S,13R,14R,17R)-3,7,12-trihydroxy-8,10,13-trimethyl-1,2,3,4,5,6,7,9,11,12,14,15,16,17-tetradecahydrocyclopenta[a]phenanthren-17-yl]pentanoate → Palmitic Acid

HRID 611460

反応式

HRID 611460 の反応式
C[C@@H:2]([C@@H:8]1[C@@:12]2(C)[C@@H:13](O)[CH2:14][C@@H:15]3[C@@:20]4(C)[CH2:21]C[C@@H](O)C[C@H:19]4[CH2:18][C@@H:17](O)[C@@:16]3(C)[C@@H:11]2[CH2:10][CH2:9]1)[CH2:3][CH2:4][C:5]([O-:7])=[O:6].[Na+]>[Cl-].[Ca+2].[Cl-]>[C:5]([OH:7])(=[O:6])[CH2:4][CH2:3][CH2:2][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][CH2:18][CH3:19].[C:5]([OH:7])(=[O:6])[CH2:4][CH2:3][CH2:2][CH2:8][CH2:9][CH2:10][CH2:11]/[CH:12]=[CH:13]\[CH2:14][CH2:15][CH2:16][CH2:17][CH2:18][CH2:19][CH2:20][CH3:21].[C:5]([OH:7])(=[O:6])[CH2:4][CH2:3][CH2:2][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][CH2:18][CH2:19][CH2:20][CH3:21]

参加物

5

反応物 1

HCID 44582063sodium (4R)-4-[(3R,5S,7R,8R,9R,10S,12S,13R,14R,17R)-3,7,12-trihydroxy-8,10,13-trimethyl-1,2,3,4,5,6,7,9,11,12,14,15,16,17-tetradecahydrocyclopenta[a]phenanthren-17-yl]pentanoate

生成物 3

HCID 985Palmitic Acid収率 47%HCID 5281Stearic AcidHCID 445639Oleic Acid

試薬・触媒・溶媒 1

HCID 5284359Calcium Chloride溶媒

実験手順

To 0.5 mL of a cultured cell fraction of Pyrococcus horikoshii JCM 9974 were added 2 mg of each of these substrates, 40 μl of 100 mM sodium cholate and 100 μL of 20 mM calcium chloride. The resultant reaction solution was reacted at 75° C. overnight. From the reaction solution, a fraction containing the fatty acid produced was extracted with isooctane and then subjected to gas chromatography. As a result, a decomposition product including 47-48% of oleic acid, oleic acid, palmitic acid or linolenic acid resulted from the decomposition by phospholipase A2 and 47-48% of palmitic acid, stearic acid, oleic acid or stearic acid resulted from the decomposition by phospholipase A1 was obtained from each of the substrates.

後処理

  1. customThe resultant reaction solution
  2. customwas reacted at 75° C. overnight
  3. customproduced
  4. extractionwas extracted with isooctane

出典と根拠

ORDレコード
ord-c8c00cdd1b30490b9b3d5e825036be32
出典データセット
uspto-grants-1999_12
特許番号
US06001626

関連ノート

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