関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|
| 反応物 | Triethylamine | HCID 8471 | — |
| 反応物 | NHS-Biotin | HCID 434213 | — |
| 反応物 | N-(6-Aminohexyl)-4-azidobenzamide | HCID 13330101 | — |
| 溶媒 | Dimethylformamide | HCID 6228 | — |
| 生成物 | Biotin [2-(4-azidobenzoyl)aminohexyl]amidE | HCID 131721358 | — |
反応手順
1.30 g (5 mmol) of product 2b are suspended in 40 ml of distilled DMF and 1.4 ml triethylamine are added under stirring at 20° C. Subsequently, 1.71 g (5 mmol) of biotin-N-hydroxysuccinimide ester 3 are added and stirring is continued for another 3 h at 20° C. Subsequently, the solvent is removed on a rotation evaporator (oil pump vacuum, water bath 50° C.) and the concentrated residue is digested overnight with saturated NaHCO3 solution. Product 4b is drawn off, washed with water and dried in the exsiccator.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| 特許番号 | US05952185 |
|---|
| 出典データセット | uspto-grants-1999_09 |
|---|
| ORD ID | ord-5565c67d56f746eb8a2c86c07a4205b6 |
|---|
| 出典メモ | uspto-grants-1999_09 |
|---|
