Uracil → D-xylonic acid
反応式
N1C=CC(=O)NC1=[O:3].O=C[C@@H:11]([C@H:13]([C@@H:15]([C@@H:17]([CH2:19][OH:20])[OH:18])[OH:16])[OH:14])[OH:12].O=C[C@@H]([C@H]([C@@H](CO)O)O)O>>[O:3]=[C:11]([OH:12])[C@@H:13]([C@H:15]([C@@H:17]([CH2:19][OH:20])[OH:18])[OH:16])[OH:14]参加物
4
実験手順
The resulting strains H3679 and H3680 were cultured on SC-medium lacking uracil for plasmid selection, with 10 g I−1 glucose and 20 g I−1 xylose as carbon sources. The strains were cultured in 250 ml Erlenmeyer flasks in 50 ml medium with 250 rpm agitation at 30° C. Strain H3679 expressing the gene CC_1225 produced 3.3 g xylonic acid I−1 extracellularly in 75 h (FIG. 14). Strain H3680 (control strain) produced no xylonic acid when grown under the same conditions.
後処理
- customwere cultured in 250 ml Erlenmeyer flasks in 50 ml medium with 250 rpm agitation at 30° C
出典と根拠
- ORDレコード
- ord-709420b040f44d23ac85a109df124b37
- 出典データセット
- uspto-grants-2016_04
- 特許番号
- US09315834B2
反応ノート
読み込み中…