化工社化工社 化学情報印刷レポート · 研究参考用
Water → Deoxyuridine Triphosphate
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Water | HCID 962 | — |
| 反応物 | 5-(3-Amino)allyldeoxyuridine 5'-triphosphate | HCID 86599380 | — |
| 溶媒 | Dimethyl Sulfoxide | HCID 679 | — |
| 溶媒 | Sodium carbonate bicarbonate | HCID 86624777 | — |
| 生成物 | Deoxyuridine Triphosphate | HCID 65070 | — |
反応手順
Dye-PC NHS ester (3 equivalents) dissolved in DMSO or other water miscible solvent is added to a solution of 5-(3-aminoallyl)-2′-deoxyuridine-5′-triphosphate in 0.1M sodium carbonate/bicarbonate buffer (pH 8.5). Mixture is stirred at room temperature for 2-3 h and crude reaction is purified by reversed-phase HPLC.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US09322051B2 |
| 出典データセット | uspto-grants-2016_04 |
| ORD ID | ord-f39235db8c08411b9ead5475f7444253 |
| 出典メモ | uspto-grants-2016_04 |