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Citric Acid → 5-Hydroxyeicosatetraenoic Acid
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Citric Acid | HCID 311 | — |
| 反応物 | 5-Hydroxyicosa-6,8,11,14-tetraenoic acid | HCID 1771 | — |
| 反応物 | Nordihydroguaretic acid | HCID 4534 | — |
| 反応物 | Arachidonic Acid | HCID 444899 | — |
| 反応物 | Calcimycin | HCID 11957499 | — |
| 生成物 | 5-Hydroxyeicosatetraenoic Acid | HCID 5280733 | — |
反応手順
A suspension of human neutrophils in buffer is incubated for 3 min at 30° C. with (14C)-arachidonic acid (AA) and calcium ionophore A23187. Citric Acid (2M) /NDGA* (10 mM) is used to quench the reaction. Following the addition of a trace amount of (3H)-5-HETE together with an excess of unlabeled 5-HETE to each tube, the mixture is extracted with chloroform/methanol. The organic layer is washed with dilute hydrochloric acid and the total volume is transferred to glass tubes and dried in vacuo. The residue is dissolved in a small volume of chloroform and is spotted on silica gel TLC sheets which are developed with an ethyl acetate/isooctane/water/acetic acid solvent system
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US04677210 |
| 出典データセット | uspto-grants-1987_06 |
| ORD ID | ord-9d92797f31cb495c9f7ebc60d4534c5c |
| 出典メモ | uspto-grants-1987_06 |