Phosphate → Glyoxal 2'-deoxyguanosine

HRID 723306

反応式

HRID 723306 の反応式
[C@@H:1]1(N2C=CC(=O)NC2=O)[O:8][C@H:5]([CH2:6][OH:7])[C@@H:3]([OH:4])[CH2:2]1.C(C=O)=O.[NH:21]1[C:30](=[O:31])[C:29]2[NH:28][CH:27]=[N:26][C:25]=2[N:24]=[C:22]1[NH2:23].P([O-])([O-])([O-])=O.B(O)(O)O.C(N(CC(O)=O)CC(O)=O)CN(CC([O-])=O)CC([O-])=O.[Na+].[Na+]>>[CH:3]([CH:5]=[O:8])=[O:4].[C@@H:1]1([N:26]2[C:25]3[N:24]=[C:22]([NH2:23])[NH:21][C:30](=[O:31])[C:29]=3[N:28]=[CH:27]2)[O:8][C@H:5]([CH2:6][OH:7])[C@@H:3]([OH:4])[CH2:2]1

参加物

6

反応物 5

HCID 1061PhosphateHCID 7628Boric AcidHCID 13712Deoxyuridine2 回HCID 13020083Ethylenediaminetetraacetic acid disodium saltHCID 136394281Glyoxal guanine2 回

生成物 1

HCID 136394279Glyoxal 2'-deoxyguanosine

実験手順

Glyoxal-2′-deoxyguanosine was synthesized from 2′-deoxyuridine and glyoxal-guanine under the following conditions, by using any one of the aforementioned three sets of enzyme solutions and the Bacillus stearothermophilus JTS 859 enzyme solution obtained in Production Example 2. Specifically, 5 mM phosphate buffer (pH 7) containing 30 mM 2′-deoxyuridine, 30 mM glyoxal-guanine, 100 mM boric acid, and 4 mM disodium ethylenediaminetetraacetate was first prepared.

後処理

  1. customthe Bacillus stearothermophilus JTS 859 enzyme solution obtained in Production Example 2
  2. customwas first prepared

出典と根拠

ORDレコード
ord-665192301b284d88b2a096ea61b8b4b6
出典データセット
uspto-grants-2006_11
特許番号
US07141397B2

関連ノート

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