Phosphoric Acid → (2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)ammonium dihydrogen phosphate

HRID 727766ノート

反応式

HRID 727766 の反応式
[CH2:1]([OH:8])[C:2]([NH2:7])([CH2:5][OH:6])[CH2:3][OH:4].[P:9](=[O:13])([OH:12])([OH:11])[OH:10]>O>[CH2:1]([OH:8])[C:2]([NH2:7])([CH2:5][OH:6])[CH2:3][OH:4].[OH:11][P:9]([OH:13])([OH:12])=[O:10]

参加物

4

反応物 2

HCID 1004Phosphoric AcidHCID 6503Tris(hydroxymethyl)aminomethane

生成物 1

HCID 3083992(2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)ammonium dihydrogen phosphate

試薬・触媒・溶媒 1

HCID 962Water溶媒2 回

実験手順

A sample of well agitated fermentation mixture (5.0 ml) is centrifuged for five minutes at 17,000 rpm in a centrifuge tube, the supernate decanted and discarded, and the pellet resuspended in 4.9 ml of 0.1M tris phosphate buffer. The tris phosphate buffer is prepared by dissolving 12.1 grams of Sigma Chem. Co. T-1503 Trizma base in 800 ml of deionized water, adjusting the pH at 25° C. to 7.5 with phosphoric acid and diluting with deionized water to a final volume of 1000 ml.

後処理

  1. customthe supernate decanted
  2. dissolutionby dissolving 12.1 grams of Sigma Chem
  3. customat 25° C.

出典と根拠

ORDレコード
ord-5b9c7780e36b4c9391c0eaef9d762d50
出典データセット
uspto-grants-1991_09
特許番号
US05045465

反応ノート

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