Bicarbonate Ion → Biotin

HRID 7341

反応式

HRID 7341 の反応式
[C:1](=[O:4])(O)[O-:2].[CH2:5]1[S:9][C@@H:8]([CH2:10][CH2:11][CH2:12][CH2:13]C(NCCCCCC(ON2C(=O)C(S([O-])(=O)=O)CC2=O)=O)=O)[C@H:7]2[NH:36][C:37]([NH:39][C@@H:6]12)=[O:38].[Na+]>O>[OH:2][C:1]([CH2:13][CH2:12][CH2:11][CH2:10][C@H:8]1[C@@H:7]2[C@@H:6]([NH:39][C:37]([NH:36]2)=[O:38])[CH2:5][S:9]1)=[O:4]

参加物

4

反応物 2

HCID 769Bicarbonate IonHCID 71571497Sulphosuccinimidyl-6-(biotinamido)hexanoate

生成物 1

HCID 171548Biotin

試薬・触媒・溶媒 1

HCID 962Water溶媒

実験手順

About 2.0 mg of DBP is buffer exchanged with 20 mM bicarbonate at pH 9.6 three times to give a final volume of 1.0 mL. Two aliquots of sulfo-NHS-LC-biotin (Pierce) are dissolved in water to give a 1 mg/mL solution just prior to addition. The first aliquot (10 uL) is added and the reaction is maintained at room temperature for 5 minutes. The second aliquot (10 uL) is added and the reaction proceeds for an additional 4 minutes. The unreacted biotin is removed by size exclusion chromatography using SEPHADEX G-25 and 100 mM PBS, pH 7.4 as the mobile phase.

後処理

  1. customto give a final volume of 1.0 mL
  2. customto give a 1 mg/mL solution just
  3. additionto addition
  4. additionThe first aliquot (10 uL) is added
  5. additionThe second aliquot (10 uL) is added
  6. customThe unreacted biotin is removed by size exclusion chromatography

出典と根拠

ORDレコード
ord-34edcaa7e3ba4173928e329654c5e7d9
出典データセット
uspto-grants-2006_08
特許番号
US07087395B1

関連ノート

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