Panose → Isomaltose

HRID 734172

反応式

HRID 734172 の反応式
[CH2:1]([OH:34])[C@H:2]1[O:7][C@H:6]([O:8][CH2:9][C@H:10]2[O:15][C@H:14]([O:16][C@@H]([C@H](O)[C@@H](O)C=O)[C@H](O)CO)[C@H:13]([OH:28])[C@@H:12]([OH:29])[C@@H:11]2[OH:30])[C@H:5]([OH:31])[C@@H:4]([OH:32])[C@@H:3]1[OH:33]>O>[CH2:1]([OH:34])[C@H:2]1[O:7][C@H:6]([O:8][CH2:9][C@H:10]2[O:15][CH:14]([OH:16])[C@H:13]([OH:28])[C@@H:12]([OH:29])[C@@H:11]2[OH:30])[C@H:5]([OH:31])[C@@H:4]([OH:32])[C@@H:3]1[OH:33]

参加物

3

反応物 1

HCID 94448Panose

生成物 1

HCID 439193Isomaltose

試薬・触媒・溶媒 1

HCID 962Water溶媒

反応条件

温度
100 °C

実験手順

To a 0.2% aqueous solution of panose, α-isomaltosylmaltose, α-isomaltosyltriose, α-isomaltosyltetraose, or cyclotetrasaccharide was added 100 units/g solid, d.s.b., of an isomaltodextranase specimen, obtained by the method in Experiment 29, where 3,000 units/g solid, d.s.b., of the specimen was also used for the aqueous solution with cyclotetrasaccharide. The mixture was subjected to an enzymatic reaction at 40° C. and pH 5.5 for 24 hours and heated at 100° C. for 20 min to suspend the enzymatic reaction. The saccharide composition of the resulting mixture was analyzed on HPLC using column of “MCIGEL CK04SS”, a column commercialized by Mitsubishi Chemical Industries, Ltd., Tokyo, Japan; an inner column temperature of 80° C.; a flow rate of 0.5 ml/min of water as an eluate; and a detector of “RI-8012”, a differential refractometer commercialized by Tosoh Corporation, Tokyo, Japan. The results are in Table 34.

後処理

  1. custom, of an isomaltodextranase specimen, obtained by the method in Experiment 29, where 3,000 units/g solid
  2. customThe mixture was subjected to an enzymatic reaction at 40° C.
  3. customthe enzymatic reaction
  4. customan inner column temperature of 80° C.

出典と根拠

ORDレコード
ord-116ecd8308bc43d79b4c552e4c31e063
出典データセット
uspto-grants-2009_09
特許番号
US07592160B2

関連ノート

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