化工社化工社 化学情報印刷レポート · 研究参考用
Glycolic Acid → Formate
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Glycolic Acid | HCID 757 | — |
| 反応物 | alpha-AMINO-3-HYDROXY-5-METHYL-4-ISOXAZOLEPROPIONIC ACID | HCID 1221 | — |
| 反応物 | Isobutyric Acid | HCID 6590 | — |
| 反応物 | Sodium Hydroxide | HCID 14798 | — |
| 反応物 | Benzalkonium Chloride | HCID 3014024 | — |
| 反応物 | Hydroxyacetate | HCID 5460308 | — |
| 生成物 | Formate | HCID 283 | — |
| 生成物 | Oxalate | HCID 71081 | — |
| 生成物 | Npc324351 | HCID 3614358 | — |
反応手順
The reaction in Example 12 was repeated., using a mixture containing glycolic acid (0.500M), AMPA (0.525M), isobutyric acid (0.100M, HPLC internal standard), flavin mononucleotide (0.01 mM), and 0.72 g of Hansenula polymorpha transformant G01 (43.0 IU glycolate oxidase and 39,880 IU catalase) which had been permeabilized by treatment with 0.2% benzalkonium chloride (Lonza Barquat OJ-50) at pH 8.3 (adjusted with 50% NaOH) and 5° C. After 1 h, the HPLC yields of glyoxylate, formate, and oxalate were 50.2%, 47.4%, and 1.1%, respectively, and 2.2% glycolate remained. The remaining permeabilized cell activity of glycolate oxidase and catalase were 95% and 105%, respectively, of their initial values.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US05559020 |
| 出典データセット | uspto-grants-1996_09 |
| ORD ID | ord-35d3ab2391ce41708cab3652593ef4d7 |
| 出典メモ | uspto-grants-1996_09 |