2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid → Fluorescein Methotrexate

HRID 77268

反応式

HRID 77268 の反応式
[CH:1]1[CH:2]=[CH:3][C:4]([C:23]([OH:25])=[O:24])=[C:5]([C:7]2[C:17]3[CH:18]=[CH:19][C:20]([OH:22])=[CH:21][C:16]=3[O:15][C:14]3[C:8]=2[CH:9]=[CH:10][C:11]([CH:13]=3)=[O:12])[CH:6]=1.[CH3:26][N:27]([C:41]1[CH:42]=[CH:43][C:44]([C:47]([NH:49][C@H:50]([C:56]([OH:58])=[O:57])[CH2:51][CH2:52][C:53]([OH:55])=[O:54])=[O:48])=[CH:45][CH:46]=1)[CH2:28][C:29]1[CH:30]=[N:31][C:32]2[N:38]=[C:37]([NH2:39])[N:36]=[C:35]([NH2:40])[C:33]=2[N:34]=1>>[CH:1]1[CH:2]=[CH:3][C:4]([C:23]([OH:25])=[O:24])=[C:5]([C:7]2[C:8]3[CH:9]=[CH:10][C:11]([OH:12])=[CH:13][C:14]=3[O:15][C:16]3[C:17]=2[CH:18]=[CH:19][C:20]([CH:21]=3)=[O:22])[CH:6]=1.[CH3:26][N:27]([C:41]1[CH:42]=[CH:43][C:44]([C:47]([NH:49][C@H:50]([C:56]([OH:58])=[O:57])[CH2:51][CH2:52][C:53]([OH:55])=[O:54])=[O:48])=[CH:45][CH:46]=1)[CH2:28][C:29]1[CH:30]=[N:31][C:32]2[N:38]=[C:37]([NH2:39])[N:36]=[C:35]([NH2:40])[C:33]=2[N:34]=1

参加物

3

反応物 2

HCID 33832-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acidHCID 126941Methotrexate

生成物 1

HCID 86761749Fluorescein Methotrexate

実験手順

2.5-5×105 cells were incubated over-night at 29° C. in 0.5 mL of culture medium containing 10 mM fluorescein-labeled methotrexate (F-MTX, Molecular Probes/Invitrogen). Labeled cells were washed in serum-free culture medium and images were recorded using a fluorescence microscope (Olympus CKX41 microscope equipped with a DP50 digital camera) using a FITC filter set.

後処理

  1. wait2.5-5×105 cells were incubated over-night at 29° C. in 0.5 mL of culture medium
  2. washLabeled cells were washed in serum-free culture medium and images
  3. customequipped with a DP50 digital camera)
  4. filtrationfilter

出典と根拠

ORDレコード
ord-ad6a8630d3f84495877d53ca549259a0
出典データセット
uspto-grants-2014_09
特許番号
US08841106B2

関連ノート

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