Hydrogen Peroxide → [(2S,3S)-5,7-dihydroxy-2-[3,4,5-trihydroxy-6-oxo-8-[(2R,3R)-3,5,7-trihydroxy-3,4-dihydro-2H-chromen-2-yl]benzo[7]annulen-1-yl]-3,4-dihydro-2H-chromen-3-yl] 3,4,5-trihydroxybenzoate

HRID 8515

反応式

HRID 8515 の反応式
[CH2:1]1[C:10]2[C:5](=[CH:6][C:7]([OH:12])=[CH:8][C:9]=2[OH:11])[O:4][C@H:3]([C:13]2[CH:18]=[C:17](O)[C:16]([OH:20])=[C:15]([OH:21])[CH:14]=2)[C@@H:2]1[O:22][C:23]([C:25]1[CH:30]=[C:29]([OH:31])[C:28]([OH:32])=[C:27]([OH:33])[CH:26]=1)=[O:24].OO>CC(C)=O>[CH2:1]1[C:10]2[C:5](=[CH:6][C:7]([OH:12])=[CH:8][C:9]=2[OH:11])[O:4][C@H:3]([C:13]2[CH:18]=[C:18]3[C:13]([C@@H:3]4[O:4][C:5]5[C:10](=[C:9]([OH:11])[CH:8]=[C:7]([OH:12])[CH:6]=5)[CH2:1][C@@H:2]4[O:22][C:23]([C:25]4[CH:26]=[C:27]([OH:33])[C:28]([OH:32])=[C:29]([OH:31])[CH:30]=4)=[O:24])=[CH:14][C:15]([OH:21])=[C:16]([OH:20])[C:17]3=[C:16]([OH:20])[C:15](=[O:21])[CH:14]=2)[C@@H:2]1[OH:22]

参加物

4

反応物 2

HCID 784Hydrogen PeroxideHCID 65064Epigallocatechin Gallate

生成物 1

HCID 102115506[(2S,3S)-5,7-dihydroxy-2-[3,4,5-trihydroxy-6-oxo-8-[(2R,3R)-3,5,7-trihydroxy-3,4-dihydro-2H-chromen-2-yl]benzo[7]annulen-1-yl]-3,4-dihydro-2H-chromen-3-yl] 3,4,5-trihydroxybenzoate収率 28.1%

試薬・触媒・溶媒 1

HCID 180Acetone溶媒

実験手順

EC (1 g) and EGCG (1 g) were dissolved in a mixture of acetone-pH 5.0 phosphate citrate buffer (1:10, v/v, 50 mL), which contained 4 mg horseradish peroxidase. While being stirred, 2.0 ml of 3.13% H2O2 was added four times during 45 minutes. The reaction mixture was extracted by ethyl acetate (50 ml×3). After concentration, the residue was subjected to Sephadex LH 20 column eluted with acetone-water solvent system (45%). 220 mg theaflavin 3-gallate was obtained.

後処理

  1. extractionThe reaction mixture was extracted by ethyl acetate (50 ml×3)
  2. concentrationAfter concentration
  3. washeluted with acetone-water solvent system (45%)

出典と根拠

ORDレコード
ord-8b2708b9760e412ba365ccc68af26b1b
出典データセット
uspto-grants-2006_08
特許番号
US07087790B2

関連ノート

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