化工社化工社 化学情報印刷レポート · 研究参考用
Leukotriene C4 → Leukotrien C4
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Leukotriene C4 | HCID 5280493 | — |
| 反応物 | methyl 4-[(2S,3S)-3-[(1E,3E,5Z,8Z)-tetradeca-1,3,5,8-tetraenyl]oxiran-2-yl]butanoate | HCID 6478221 | — |
| 反応物 | Serine borate | HCID 18648594 | — |
| 溶媒 | Phosphate | HCID 1061 | — |
| 生成物 | Leukotrien C4 | HCID 44563898 | — |
反応手順
LTC4 synthase prepared as a crude fraction from guinea pig lung was used. The test compounds7were tested in duplicate at a concentration of 300 μg/ml. The test compound and/or vehicle was incubated with 12 μg enzyme, 0.3 μg LTA4 methyl ester, 0.2% (w/w) albumin (to stabilize the product) and 4.5 mM serine borate (to prevent conversion of LTC4 to LTD4) in phosphate buffer pH 7.8 for 30 minutes at 37° C. The reaction was terminated by addition of ice-cold methanol. Formation of LTC4 was quantitated by RIA (radioimmunoassay).
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US07252845B2 |
| 出典データセット | uspto-grants-2007_08 |
| ORD ID | ord-1cfbfd07b15548a49aa5a01e196e25f6 |
| 出典メモ | uspto-grants-2007_08 |