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Hydrochloric Acid → Phthalaldehyde
関与物質
| 役割 | 名称 | HCID | 仕込み量 |
|---|---|---|---|
| 反応物 | Hydrochloric Acid | HCID 313 | — |
| 反応物 | L-Phenylalanine | HCID 6140 | — |
| 反応物 | tert-Butyloxycarbonyl-phenylalanyl-leucyl-phenylalanyl-leucyl-phenylalanyl-OH | HCID 132822 | — |
| 反応物 | o-Phthalaldehyde 2-mercaptoethanol | HCID 88526415 | — |
| 溶媒 | Methanol | HCID 887 | — |
| 生成物 | Phthalaldehyde | HCID 4807 | — |
反応手順
Four aliquots of the conjugate were hydrolyzed with concentrated HCl at 115° C. for 20 hours and analyzed for phenylalanine. The phenylalanine was quantified as the o-phthalaldehyde/2-mercaptoethanol derivative using reverse phase HPLC with a fluorescence detector (HPLC conditions as Example 24 except the eluent gradient was 12% to 80% methanol over 20 min. and then held at 80% for 5 min.). The o-phthalaldehyde derivative was prepared as given in Example 24. For a 25 μL aliquot of the conjugate, 26 nmoles phenylalanine were found. This corresponds to 0.26 μmoles BOCPheLeuPheLeuPhe on the conjugate.
情報源と証拠
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 特許番号 | US05714166 |
| 出典データセット | uspto-grants-1998_02 |
| ORD ID | ord-14382bf0085c49fea90fd46467495ff8 |
| 出典メモ | uspto-grants-1998_02 |