Dinoprost → 8-Epi-prostaglandin F2alpha

HRID 868799ノート

反応式

HRID 868799 の反応式
[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][C@H:6]([OH:25])/[CH:7]=[CH:8]/[C@@H:9]1[C@@H:13]([CH2:14]/[CH:15]=[CH:16]\[CH2:17][CH2:18][CH2:19][C:20]([OH:22])=[O:21])[C@@H:12]([OH:23])[CH2:11][C@H:10]1[OH:24]>C(O)C>[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][C@H:6]([OH:25])/[CH:7]=[CH:8]/[C@@H:9]1[C@H:13]([CH2:14]/[CH:15]=[CH:16]\[CH2:17][CH2:18][CH2:19][C:20]([OH:22])=[O:21])[C@@H:12]([OH:23])[CH2:11][C@H:10]1[OH:24]

参加物

3

反応物 1

HCID 5280363Dinoprost

生成物 1

HCID 52822638-Epi-prostaglandin F2alpha

試薬・触媒・溶媒 1

HCID 702Ethanol溶媒

実験手順

All isomers of PGF2α were dissolved or diluted in adequate amount of ethanol and after aliquot, stored at −70° C. As an internal standard, 10 ng of 8-iso-PGF2α-d4 was mixed with 0.5 ml of plasma in a glass tube and was then purified by a C18 Solid-Phase Extraction column (J. T. Baker, MA, USA). Sample eluants were evaporated under a stream of nitrogen and re-dissolved in 50 μl acetonitrile:water (15:85 v/v) solution followed by vortex for 30 seconds and 10 μl extract was further analyzed with a LC/MS/MS system.

後処理

  1. customstored at −70° C
  2. additionAs an internal standard, 10 ng of 8-iso-PGF2α-d4 was mixed with 0.5 ml of plasma in a glass tube
  3. customwas then purified by a C18 Solid-Phase Extraction column (J. T. Baker, MA, USA)
  4. customSample eluants were evaporated under a stream of nitrogen
  5. dissolutionre-dissolved in 50 μl acetonitrile:water (15:85 v/v) solution
  6. extraction10 μl extract

出典と根拠

ORDレコード
ord-5d36c153762c4aa281bee7774353d32e
出典データセット
uspto-grants-2015_03
特許番号
US08969312B2

反応ノート

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