Ethanol → Ethinylestradiol

HRID 879905

反応式

HRID 879905 の反応式
[CH3:1][C@@:2]12[C@@H:18]([OH:19])[CH2:17][CH2:16][C@H:15]1[C@H:14]1[C@@H:5]([C:6]3[CH:7]=[CH:8][C:9]([OH:20])=[CH:10][C:11]=3[CH2:12][CH2:13]1)[CH2:4][CH2:3]2.[CH2:21](O)[CH3:22]>P([O-])([O-])([O-])=O>[CH3:1][C@@:2]12[C@:18]([OH:19])([C:21]#[CH:22])[CH2:17][CH2:16][C@H:15]1[C@H:14]1[C@@H:5]([C:6]3[CH:7]=[CH:8][C:9]([OH:20])=[CH:10][C:11]=3[CH2:12][CH2:13]1)[CH2:4][CH2:3]2

参加物

4

反応物 2

HCID 702EthanolHCID 5757Estradiol

生成物 1

HCID 5991Ethinylestradiol

試薬・触媒・溶媒 1

HCID 1061Phosphate溶媒

反応条件

温度
37 °C

実験手順

Estradiol conjugate (33.5 mg, 0.039 mmol), prepared from Conjugate 1 as described in Example 16, was dissolved in 58 mL of a 9:4 ethanol/0.05 M aqueous phosphate buffer (pH 7.4) mixture, and the solution was shaken in a 37° C. incubator for 9 days. Six 8 mL aliquots were collected at 1-, 2-, 3-, 5-, 7-, and 9-day intervals. Estradiol release was quantified by adding 33 μL of a 0.09 M solution of butylated hydroxytoluene (BHT) in deuterochloroform as an internal standard, then comparing integrals of 1H NMR signals corresponding to the steroid and the internal standard. Results are shown in Table 4 below.

後処理

  1. customSix 8 mL aliquots were collected at 1-, 2-, 3-, 5-, 7-, and 9-day intervals
  2. additionby adding 33 μL of a 0.09 M solution of butylated hydroxytoluene (BHT) in deuterochloroform as an internal standard
  3. customResults

出典と根拠

ORDレコード
ord-7a8a9a94b78c43e189ad97367162b27d
出典データセット
uspto-grants-2015_03
特許番号
US08992977B2

関連ノート

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