에티듐 브로마이드

에티듐브로마이드 · 에티듐브롬화물 · 에티듐브롬화물용액 · 에티듐브롬화물(ETHIDIUMBROMIDE) · 에티듐 브로마이드 · ETBR · Ethidium bromide solution · HOMIDIUM BROMIDE

https://huagongshe.com/ko/chemical/14710

식별자 및 물리적 특성

CAS No.	1239-45-8
분자식	C21H20N3.Br
평균 분자량	394.3 g/mol
단동위원소 질량	393.08405974 g/mol
SMILES	CC[n+]1c(-c2ccccc2)c2cc(N)ccc2c2ccc(N)cc21.[Br-]
InChIKey	ZMMJGEGLRURXTF-UHFFFAOYSA-N
PubChem CID	14710
ChEMBL	CHEMBL284328
ChEBI	CHEBI:4883
EC No.	214-984-6
DTXSID	DTXSID8025258
현지명	에티듐 브로마이드

화학 및 물리적 성질

극성 표면적	55.9
수소 결합 공여자 수	2
수소 결합 수용자 수	3
회전 가능 결합 수	2
무거운 원자 수	25
형식 전하	0
복잡도	419
물질 분류	Nitrogen Compounds -> Other Aromatics (Nitrogen)
LogP(분배 계수)	log Kow= -0.38 /Estimated/-1.1
맛	Bitter tasting
밀도	0.34 g/cm³1.3739 (rough estimate)
Color/Form	Dark red crystals from alcoholMaroon powder
용해도	Solubility in 2-methoxyethanol is 20 mg/mLethanol, 2 mg/mLSoluble in 750 parts chloroform at 20 °CSoluble in 20 parts water at 20 °CSolubility in water, g/100ml at 20 °C: 5H2O: 10 mg/mL, 불투명, 강한 빨간색
인화점	>100 °C>100°C
Decomposition	When heated to decomposition it emits very toxic fumes of /hydrogen bromide and nitrogen oxides/.
녹는점	260-262 °C (decomposes)238-240 °C260-262 °C (dec.)(lit.)
증기압	1.2X10-12 mm Hg at 25 °C /Estimated/Vapor pressure at 25 °C: negligible
헨리 상수	Henry's Law constant= 1.8X10-20 atm-cu m/mole at 25 °C /Estimated/
Physical Description	Dark red solid[Merck Index] Red to brown solid[ICSC] Dark red powder[Sigma-Aldrich MSDS]RED-TO-BROWN CRYSTALS.
굴절률	1.6700 (estimate)
저장 조건	2-8°C
물리적 상태	가루
색상	빨간색에서 진한 보라색까지
pH	4.4 (20°C, 10g/L in H2O)
냄새	무취 고체
용해도	40g/L(25℃)
Merck	14,4731
BRN	3642536
안정성	안정적인. 강한 산화제와 호환되지 않습니다.
주요 응용	전광 디스플레이포토레지스트
InChI	1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,23H,2,22H2,1H3
InChIKey	ZMMJGEGLRURXTF-UHFFFAOYSA-N
SMILES	[Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
EPA	Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide (1239-45-8)

화학적 성질

Purple/maroon crystalline powder

안전 및 규제

GHS 분류	DangerH302: Harmful if swallowed [Warning Acute toxicity, oral]P203, P260, P264, P270, P271, P280, P284, P301+P317, P304+P340, P316, P318, P320, P330, P403+P233, P405, and P501 (click each P-code to see the statement)H302 (97.1%): Harmful if swallowed [Warning Acute toxicity, oral]P203, P260, P261, P264, P270, P271, P280, P284, P301+P317, P304+P340, P316, P318, P320, P321, P330, P403+P233, P405, and P501 (click each P-code to see the statement)
규제 정보	Chemical: Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromideEthidium bromide: HSNO Approval: HSR004464 Approved with controls
기타 안전 정보	IMAP assessments - Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide: Human health tier I assessment
화재 위험	Combustible. Gives off irritating or toxic fumes (or gases) in a fire.
유해성 요약	A skin, eye, and respiratory tract irritant[ICSC] May cause irritationToxic by ingestionHighly toxic by inhalationMutagenic effects observed in vitro[Sigma-Aldrich MSDS] See Propidium iodide.
UN 분류	UN Hazard Class: 6.1UN Pack Group: I
Chemical Dangers	Decomposes on heating. This produces toxic gases including hydrogen bromide (see ICSC 0282) and nitrogen oxides (see ICSCs 0930 and 1311). Reacts violently with strong oxidants.
Packaging and Labelling	Do not transport with food and feedstuffs.
Hazard Classes and Categories	Acute Tox. 4 (97.1%)Germ cell mutagenicity - category 2Muta. 2
Shipment Methods and Regulations	Employers /must/ ensure that labels on incoming containers of hazardous chemicals are not removed or defaced. Employers /must/ maintain any material safety data sheets that are received with incoming shipments of hazardous chemicals, and ensure that they are readily accessible to laboratory employees.

용도

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. Antiprotozoal (Trypanosoma).