7-Aminoactinomycin D

개요7-Aminoactinomycin D (7-AAD) is a fluorescent DNA dye that is commonly used for the detection or exclusion of non-viable cells in flow cytometric analysis, as it is generally excluded by live cells. It displays excitation spectra of 488, 546, and 578 nm and an emission spectrum of 650 nm. As 7-AAD is detected in the far red range of the spectrum, it exhibits minimal spectral overlap with commonly used probes, therefore it can be used in conjunction with probes such as FITC. 7-AAD has been used to evaluate apoptosis and cell-mediated cytotoxicity and to stain DNA in cells that have been fixed and permeabilized by a variety of methods.

용도Fluorescent DNA stain. A featured product for Apoptosis research.

일반 설명Free form: Excitation: 503 nm (0.01 M phosphate buffer, pH 7.0 containing 0.1 mM EDTA); 550 nmEmission: 675 nm (0.01 M phosphate buffer, pH 7.0 containing 0.1 mM EDTA); 672 nmDNA Complex: Excitation: 543 nm; 555 nmEmission: 655 nm; 665 nm