아스코르베이트옥시데이트
아스코르베이트옥시데이트 · ASO · AOX · Ascorbate Oxidase from microorganism · ASOD · C:C&B8 · ASO-311 · ASCORBASE
식별자 및 물리적 특성
| CAS No. | 9029-44-1 |
|---|---|
| 분자식 | C35H36N6O3 |
| 평균 분자량 | 588.7 g/mol |
| 단동위원소 질량 | 588.28488903 g/mol |
| SMILES | Cc1c2n(C)c3ccc(cc13)CCC=CCCn1nnc3c(C)c(cnc31)C(CC(=O)O)c1ccc3c(c1)CN(CC3)C2=O |
| InChIKey | IJDQXSQWJLQCRL-UHFFFAOYSA-N |
| PubChem CID | 168009863 |
| EC No. | 232-852-6 |
| 현지명 | 아스코르베이트옥시데이트 |
화학 및 물리적 성질
| XLogP | 5.1 |
|---|---|
| 극성 표면적 | 106 |
| 수소 결합 공여자 수 | 1 |
| 수소 결합 수용자 수 | 6 |
| 회전 가능 결합 수 | 2 |
| 무거운 원자 수 | 44 |
| 형식 전하 | 0 |
| 복잡도 | 1,090 |
| 저장 조건 | 2-8°C |
| 용해도 | 수성 완충액에 용해됩니다. |
| 물리적 상태 | 가루 |
| 색상 | 희미하게 푸르스름한 |
| 생물학적 소스 | plant (Cucurbita spp.) |
| Specific Activity | 1,000-3,000units/mg protein |
화학적 성질
Appearance : Light blue amorphous powder, lyophilized Stability : Store at －20℃ (A decrease in activity of ca.20% may occur at 5℃ within 6 months.) Molecular weight : 132,000, 140,000 Isoelectric point : between 6.0 and 7.8 , 8.2 Michaelis constant : 2.5×10－4M (Ascorbate) Structure : 8 copper atoms per mol of enzyme Inhibitors : cyanide, Na2S, diethyldithiocarbamate (Na) Optimum pH : 5.6 Optimum temperature : approx. 30℃ pH Stability : pH 7.0－10.0 (25℃, 17hr) Thermal stability : below 40℃ (pH 8.0, 30min) Substrate specificity : This enzyme oxidizes ascorbic acid and several ascorbic derivatives.
안전 및 규제
| GHS 분류 | This chemical does not meet GHS hazard criteria for 14.3% (1 of 7) of reports.DangerH334 (85.7%): May cause allergy or asthma symptoms or breathing difficulties if inhaled [Danger Sensitization, respiratory]P233, P260, P271, P284, P304+P340, P342+P316, P403, and P501 (click each P-code to see the statement)Aggregated GHS information provided per 7 reports by companies from 2 notifications to the ECHA C&L Inventory. |
|---|---|
| 규제 정보 | Oxidase, ascorbate: Does not have an individual approval but may be used under an appropriate group standard |
| Hazard Classes and Categories | Resp. Sens. 1 (85.7%) |
| 수질 위해 등급(WGK) | 3 |
| 관세 코드 | 35079090 |
| 안전 문구(S) | 24/25 |
| 기존화학 물질 | KE-01946 |
| F 고인화성물질 | 10-21 |
| 위험 등급 | Resp. Sens. 1 |
| 저장 분류 | 11 - Combustible Solids |
용도
용도
Ascorbate oxidase, from Cucurbita sp., may be used to study oxidative stress and heat stress response and tolerance. Also used in ascorbic acid assays to study the heat stress response of Arabidopsis and useful for enzymatic determination of ascorbic acid and for eliminating the interference of ascorbic acid in clinical analysis.
일반 설명
Ascorbate oxidase is a homodimeric enzyme, which comprises 552 amino acid residues per subunit (zucchini). It corresponds to a molecular mass of 70kDa per subunit. This enzyme is mainly found in plants, fungi and eubacteria.