Huagongshe화공사 화학 정보 인쇄 보고서 · 연구 참고용
엔터키나제
엔터키나제 · RECOMBINANT ENTEROKINASE · EC 3.4.21.9 · ENTEROPEPTIDASE · REK · ENTK · PRSS7 · TMPRSS15
식별자 및 물리적 특성
| CAS No. | 9014-74-8 |
|---|---|
| 분자식 | C148H230F2O26S8 |
| 평균 분자량 | 2,719.9 g/mol |
| 단동위원소 질량 | 2,717.4409131 g/mol |
| SMILES | CC(C)(C)C=CC(CCCCS(=O)(=O)O)Cc1ccc(F)cc1.CC(C)(C)C=CC(CCCCS(=O)(=O)O)Cc1ccc(F)cc1.CC(C)(C)C=CC(CCCCS(=O)(=O)O)Cc1ccccc1.CC(C)(C)C=CC(CCCCS(=O)(=O)O)Cc1ccccc1.CC(C)(C)C=CC(CCCCS(=O)(=O)O)c1ccccc1.CC(C)CC(C=Cc1ccc(C(C)(C)C)cc1)CCCCS(=O)(=O)O.COc1ccc(CC(C=CC(C)(C)C)CCCCS(=O)(=O)O)cc1.COc1ccc(CC(C=CC(C)(C)C)CCCCS(=O)(=O)O)cc1 |
| InChIKey | VRASXGTWKFQRNS-UHFFFAOYSA-N |
| PubChem CID | 168009870 |
| EC No. | 232-761-1 |
| 현지명 | 엔터키나제 |
화학 및 물리적 성질
| 극성 표면적 | 520 |
|---|---|
| 수소 결합 공여자 수 | 8 |
| 수소 결합 수용자 수 | 28 |
| 회전 가능 결합 수 | 74 |
| 무거운 원자 수 | 184 |
| 형식 전하 | 0 |
| 복잡도 | 2,230 |
| 저장 조건 | -20°C |
| 물리적 상태 | 무염, 동결건조 분말 |
| 색상 | 하얀색 |
| 생물학적 소스 | bovine intestine |
| Specific Activity | ≥20units/mg protein |
| EPA | Peptidase, entero- (9014-74-8) |
안전 및 규제
| GHS 분류 | DangerH315 (100%): Causes skin irritation [Warning Skin corrosion/irritation]P233, P260, P261, P264, P264+P265, P271, P280, P284, P302+P352, P304+P340, P305+P351+P338, P319, P321, P332+P317, P337+P317, P342+P316, P362+P364, P403, P403+P233, P405, and P501 (click each P-code to see the statement)Aggregated GHS information provided per 40 reports by companies from 3 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies. |
|---|---|
| 규제 정보 | XU - indicates a substance exempt from reporting under the Chemical Data Reporting Rule, (40 CFR 711). |
| Hazard Classes and Categories | Skin Irrit. 2 (100%) |
| 수질 위해 등급(WGK) | 3 |
| TSCA 등재 상태 | TSCA listed |
| 저장 분류 | 11 - Combustible Solids |
용도
용도
Typical conditions for fusion protein cleavage:Adjust the concentration of the fusion protein to 1.5 mg/ml and a pH between 7.0-8.0 with 500 mM Tris-HCl, pH 8.0, 2.0 mM CaCl2, and 1% Tween 20 Add enterokinase to fusion protein solution at a ratio of ~ 0.02 units per 1 mg fusion protein and mixIncubate reaction mixture at ~25 °C for 16 hours
일반 설명
Enterokinase is a highly specific serine protease that is used for the removal of the FLAG peptide from N-terminal and Met-N-terminal fusion proteins. It does not remove the C-terminal FLAG.