광택이나는 BLUE G-250
광택이나는BLUEG-250 · BRILLIANT BLUE · BRILLIANT BLUE G · COOMASSIE BRILLIANT BLUE G-250 · G-250 · COOMASSIE BRILLIANT BLUE G · CI 42655 · CBB G-250
식별자 및 물리적 특성
| CAS No. | 6104-58-1 |
|---|---|
| 분자식 | C47H48N3NaO7S2 |
| 평균 분자량 | 854 g/mol |
| 단동위원소 질량 | 853.28313751 g/mol |
| SMILES | CCOc1ccc(Nc2ccc(C(=C3C=CC(=[N+](CC)Cc4cccc(S(=O)(=O)[O-])c4)C=C3C)c3ccc(N(CC)Cc4cccc(S(=O)(=O)[O-])c4)cc3C)cc2)cc1.[Na+] |
| InChIKey | RWVGQQGBQSJDQV-UHFFFAOYSA-M |
| PubChem CID | 61363 |
| ChEMBL | CHEMBL4173394 |
| EC No. | 228-058-4 |
| 현지명 | 광택이나는 BLUE G-250 |
화학 및 물리적 성질
| 극성 표면적 | 159 |
|---|---|
| 수소 결합 공여자 수 | 1 |
| 수소 결합 수용자 수 | 9 |
| 회전 가능 결합 수 | 13 |
| 무거운 원자 수 | 60 |
| 형식 전하 | 0 |
| 복잡도 | 1,610 |
| 물질 분류 | Dyes -> Di- and Triaryl Dyes |
| 용해도 | 1mg/mLH2O: 용해성1mg/mL |
| Human Drugs | FDA approved drugs -> Active ingredient |
| Physical Description | Blue crystals[MSDSonline] |
| 녹는점 | 100 °C |
| 밀도 | 1.274[at 20℃] |
| 증기압 | 0.093Pa at 20℃ |
| 인화점 | 11 °C |
| 저장 조건 | 2-8°C |
| 물리적 상태 | 고체 |
| 색상 | 진한 파란색-보라색-갈색 |
| pH | 6.4 (20°C, 10g/L in H2O) |
| 용해도 | 물(50mg/ml)과 에탄올(40mg/ml)에 용해됩니다. |
| BRN | 5230822 |
| 안정성 | 감광성 |
| InChIKey | JFNOCRSXNFZMNT-UHFFFAOYSA-M |
| SMILES | [Na+].[S](=O)(=O)([O-])c1cc(ccc1)CN(CC)c2cc(c(cc2)[C+](c5c(cc(cc5)N(CC)Cc6cc(ccc6)[S](=O)(=O)[O-])C)c3ccc(cc3)Nc4ccc(cc4)OCC)C |
| LogP(분배 계수) | -2.55 at 20℃ |
| EPA | C.I. Acid Blue 90 (6104-58-1) |
화학적 성질
dark blue-violet-brown crystalline powder
안전 및 규제
| GHS 분류 | This chemical does not meet GHS hazard criteria for 49.3% (101 of 205) of reports.H411 (50.7%): Toxic to aquatic life with long lasting effects [Hazardous to the aquatic environment, long-term hazard]P273, P391, and P501 (click each P-code to see the statement)Aggregated GHS information provided per 205 reports by companies from 3 notifications to the ECHA C&L Inventory. |
|---|---|
| 규제 정보 | Chemical: Benzenemethanaminium, N-[4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]-2-methylphenyl]methylene]-3-methyl-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-, hydroxide, inner salt, monosodium saltREACH registered substance: Hydrogen [4-[4-(p-ethoxyanilino)-4'-[ethyl(m-sulphonatobenzyl)amino]-2'-methylbenzhydrylene]-3-methylcyclohexa-2,5-dien-1-ylidene](ethyl)(m-sulphonatobenzyl)ammonium, monosodium salt (EC: 228-058-4)Benzenemethanaminium, N-4-4-(4-ethoxyphenyl)aminophenyl4-ethyl(3-sulfophenyl)methylamino-2-methylphenylmethylene-3-methyl-2,5-cyclohexadien-1-ylidene-N-ethyl-3-sulfo-, inner salt, monosodium salt: Does not have an individual approval but may be used under an appropriate group standard |
| 유해성 요약 | A skin, eye, and respiratory tract irritant[MSDSonline] |
| Hazard Classes and Categories | Aquatic Chronic 2 (50.7%) |
| Drug Induced Liver Injury | Drug Induced Liver Injury Rank (DILIrank 2.0)Brilliant blue gvNo-DILI-concernNo matchDOI:10.1016/j.drudis.2016.02.015 |
| 수질 위해 등급(WGK) | 3 |
| TSCA 등재 상태 | TSCA listed |
| 관세 코드 | 32041200 |
| 기존화학 물질 | KE-06368 |
| 저장 분류 | 11 - Combustible Solids |
용도
용도
Brilliant Blue G is used in analytical biochemistry dye and as a P2X7 antagonist. It is also used in protein staining following gel electrophoresis. It is used to prepare the protein reagent for the determination of protein content of the collagenase enzyme isolated from fish waste. It may be employed as stain for the internal limiting membrane (ILM) for the macular hole (MH) and epiretinal membrane (ERM) surgery. It is useful for SDS gels as it readily stains proteins with minimal background color. Protein bands can be visualized during staining. It has been used in the Bradford dye-binding protein assay. A mechanism for dye binding to protein has been proposed, based on measuring Coomassie Brilliant Blue G-250 (CBBG) absorbance spectra during titration of the dye reagent in the absence of protein and its response to different polyamino acids. Brilliant Blue G has been used for determining critical micelle concentration of detergents.
일반 설명
Brilliant Blue G (BBG) is a dye. It has been proposed as a substitute for internal limiting membrane (ILM) peeling.
제조 및 합성 방법
제조 방법
Benzaldehyde (1 Moore) and N-ethyl-N-benzyl-3-methylaniline(2 Moore) condensation, And then sulfonating the product of three acids, and lead oxide, peroxide, and then the reaction with the 4-Ethoxybenzenamine, Benzaldehyde sulfonic acid groups on the phenyl ring is substituted anilino-ethoxy.