N,N-비스(2 하이드록시에틸)-2-아미노에탄설폰 산
N,N-비스(2하이드록시에틸)-2-아미노에탄설폰산 · N,N-비스(2하이드록시에틸)-2-아미노에탄설폰산(N,N-BIS(2HYDROXYETHYL)-2-AMINO... · N,N-BIS(2-HYDROXYETHYL)-2-AMINOETHANESULFONIC ACID · N,N-Bis(2-hydroxyethyl)-2-aminoethanesulphonic acid >99% · BES · abs9128 · Nsc166667 · BES, ≥99%(T)
식별자 및 물리적 특성
| CAS No. | 10191-18-1 |
|---|---|
| 분자식 | C6H15NO5S |
| 평균 분자량 | 213.25 g/mol |
| 단동위원소 질량 | 213.06709358 g/mol |
| SMILES | O=S(=O)(O)CCN(CCO)CCO |
| InChIKey | AJTVSSFTXWNIRG-UHFFFAOYSA-N |
| PubChem CID | 73243 |
| ChEBI | CHEBI:39041 |
| EC No. | 233-465-5 |
| DTXSID | DTXSID8064985 |
| 현지명 | N,N-비스(2 하이드록시에틸)-2-아미노에탄설폰 산 |
화학 및 물리적 성질
| XLogP | -4.5 |
|---|---|
| 극성 표면적 | 106 |
| 수소 결합 공여자 수 | 3 |
| 수소 결합 수용자 수 | 6 |
| 회전 가능 결합 수 | 7 |
| 무거운 원자 수 | 13 |
| 형식 전하 | 0 |
| 복잡도 | 204 |
| 물질 분류 | Other Uses -> Biological Buffers |
| Physical Description | White powder[Alfa Aesar MSDS] |
| 녹는점 | 150-155 °C |
| 밀도 | 1.210 (estimate) |
| 증기압 | 0.001Pa at 25℃ |
| 굴절률 | 1.5500 (estimate) |
| 저장 조건 | room temp |
| 용해도 | H2O: 1 M at 20 °C, 투명, 무색 |
| 물리적 상태 | 가루 |
| 색상 | 하얀색 |
| pH | 2.5-5.0 (25℃, 1M in H2O) |
| pKa(산도 계수) | 7.1(at 25℃) 7.15(at 20℃) |
| 용해도 | 녹는 |
| BRN | 1781572 |
| InChI | 1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12) |
| InChIKey | AJTVSSFTXWNIRG-UHFFFAOYSA-N |
| SMILES | OCCN(CCO)CCS(O)(=O)=O |
| LogP(분배 계수) | -3.59 at 26℃ |
| NIST | N,n-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid(10191-18-1) |
| EPA | Ethanesulfonic acid, 2-[bis(2-hydroxyethyl)amino]- (10191-18-1) |
| Absorption | ≤0.080 at 280 in H2O at 1M≤0.095 at 260 in H2O at 1M |
화학적 성질
White/clear cryst. powder
안전 및 규제
| GHS 분류 | This chemical does not meet GHS hazard criteria for 25.8% (25 of 97) of reports.WarningH315 (74.2%): Causes skin irritation [Warning Skin corrosion/irritation]P261, P264, P264+P265, P271, P280, P302+P352, P304+P340, P305+P351+P338, P319, P321, P332+P317, P337+P317, P362+P364, P403+P233, P405, and P501 (click each P-code to see the statement)Aggregated GHS information provided per 97 reports by companies from 10 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies. |
|---|---|
| 규제 정보 | Chemical: Ethanesulfonic acid, 2-[bis(2-hydroxyethyl)amino]-Status: Active Update: 23-03-2018 https://echa.europa.eu/registration-dossier/-/registered-dossier/22944Ethanesulfonic acid, 2-bis(2-hydroxyethyl)amino-: Does not have an individual approval but may be used under an appropriate group standard |
| 유해성 요약 | A skin and strong eye irritant[Alfa Aesar MSDS] |
| Hazard Classes and Categories | Skin Irrit. 2 (74.2%) |
| 수질 위해 등급(WGK) | 3 |
| TSCA 등재 상태 | TSCA listed |
| RTECS 번호 | KI7363500 |
| 관세 코드 | 29221980 |
| 위험 문구(R) | 36/37/38 |
| 위험 표시 | Xi |
| 안전 문구(S) | 26 |
| 저장 분류 | 11 - Combustible Solids |
용도
개요
BES is a zwitterionic buffer that is used in biochemistry and molecular biology research. It is one of the "Good" buffers developed in the 1960's to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. The pioneering publication by Good and co-workers describes the synthesis of BES and its physical properties.A protocol has been reported for the use of BES buffered saline in the calcium phosphate mediated transfection of eukaryotic cells with plasmid DNA. An investigation has been published on the interaction of BES and other amine buffers with DNA and the measurement of these complexes by free solution capillary electrophoresis.The effect of BES and other zwitterionic buffers on the bicinchoninic acid (BCA) measurement of microgram quantities of protein has been studied. BES was found to diminish color development of the proteinBCA complex by 30-35% at 50 mM, and by <10% at 5 mM, measuring in the 1-10 mg range of BSA against protein-BCA complexes formed in water. This color loss may result from binding of Cu2+ to BES.
용도
BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: <0.05 at 260 nm, 100 mM). Chen and Okayama describe an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The slow formation appears to be the most critical factor contributing to the high efficiency of stable transformations. Has a pKa of 7.12 at 20°C. Absorbance (100 mM, 260 nm):≤0.05.BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. Chen and Okayama described an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations.
일반 설명
BES (N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) is a useful secondary standard biochemical buffer. Useful pH range for BES is 6.4 to 7.8. It is useful for diagnostic assay manufacturing industry. BES, a sulfonic acid-containing cross-linking agent, induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes.
제조 및 합성 방법
제조 방법
First, sodium vinyl sulfonate and diethanolamine synthesize N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium by addition reaction. Through the strong acid ion exchange resin sodium ions, the eluent concentrated after adding methanol crystallization. Finally, BES is obtained after precipitation.