N-(트리스(하이드록시메틸)메틸)글리신
N-(트리스(하이드록시메틸)메틸)글리신 · N-(트리스(하이드록시메틸)메틸)글리신(N-(TRIS(HYDROXYMETHYL)METHYL)GLYCINE) · TRIS-HCL BUFFER · 1389475 · TROMETHAMINE HYDROCHLORIDE · Ticine · Tricine buffer, free acid · N-[TRIS(HYDROXYMETHYL)METHYL]GLYCINE
식별자 및 물리적 특성
| CAS No. | 5704-04-1 |
|---|---|
| 분자식 | C6H13NO5 |
| 평균 분자량 | 179.17 g/mol |
| 단동위원소 질량 | 179.07937252 g/mol |
| SMILES | O=C(O)CNC(CO)(CO)CO |
| InChIKey | SEQKRHFRPICQDD-UHFFFAOYSA-N |
| PubChem CID | 79784 |
| ChEBI | CHEBI:39063 |
| EC No. | 227-193-6 |
| DTXSID | DTXSID1063982 |
| 현지명 | N-(트리스(하이드록시메틸)메틸)글리신 |
화학 및 물리적 성질
| XLogP | -5.1 |
|---|---|
| 극성 표면적 | 110 |
| 수소 결합 공여자 수 | 5 |
| 수소 결합 수용자 수 | 6 |
| 회전 가능 결합 수 | 6 |
| 무거운 원자 수 | 12 |
| 형식 전하 | 0 |
| 복잡도 | 137 |
| Physical Description | Solid[Merck Index] White crystals[Sigma-Aldrich MSDS] |
| 녹는점 | 186-188 °C (dec.) |
| 끓는 점 | 311.69°C (rough estimate) |
| 밀도 | 1.05 g/mL at 20 °C |
| 증기압 | 0.002-0.004Pa at 20-25℃ |
| 굴절률 | 1.4240 (estimate) |
| 저장 조건 | Keep in dark place,Inert atmosphere,Room temperature |
| 용해도 | H2O: 4 M at 20 °C, 투명, 무색 |
| 물리적 상태 | 수정 같은 |
| 색상 | 투명한 무색(H2O에서 40 %(w/w)) 용액 |
| 냄새 | 냄새 없는 |
| pH | 4.0-6.0 (1M in H2O) |
| pKa(산도 계수) | 8.1(at 25℃) |
| 생물학적 소스 | synthetic |
| 용해도 | 녹는 |
| Merck | 14,9651 |
| BRN | 1937804 |
| 안정성 | 안정적인. |
| InChI | 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12) |
| InChIKey | SEQKRHFRPICQDD-UHFFFAOYSA-N |
| SMILES | OCC(CO)(CO)NCC(O)=O |
| LogP(분배 계수) | -2.87 at 25℃ |
| EPA | Glycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]- (5704-04-1) |
| Absorption | ≤0.05 at 280 in H2O at 1M≤0.1 at 260 in H2O at 1M |
화학적 성질
White/clear crystalline powder
안전 및 규제
| GHS 분류 | This chemical does not meet GHS hazard criteria for 97.6% (121 of 124) of all reports.Not ClassifiedReported as not meeting GHS hazard criteria by 121 of 124 companies (only 2.4% companies provided GHS information). For more detailed information, please visit ECHA C&L website.Aggregated GHS information provided per 124 reports by companies from 4 notifications to the ECHA C&L Inventory.Reported as not meeting GHS hazard criteria per 121 of 124 reports by companies.There are 3 notifications provided by 3 of 124 reports by companies with hazard statement code(s).Information may vary between notifications depending on impurities, additives, and other factors. The percentage value in parenthesis indicates the notified classification ratio from companies that provide hazard codes. Only hazard codes with percentage values above 10% are shown. For more detailed information, please visit ECHA C&L website. |
|---|---|
| 규제 정보 | Chemical: Glycine, N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]-Status: Active Update: 02-03-2021 https://echa.europa.eu/registration-dossier/-/registered-dossier/25731Glycine, N-2-hydroxy-1,1-bis(hydroxymethyl)ethyl-: Does not have an individual approval but may be used under an appropriate group standard |
| 기타 안전 정보 | Evaluation - Chemicals that are unlikely to require further regulation to manage risks to environment |
| 유해성 요약 | May cause irritation[Sigma-Aldrich MSDS] |
| Hazard Classes and Categories | Not Classified |
| 수질 위해 등급(WGK) | 3 |
| TSCA 등재 상태 | TSCA listed |
| 관세 코드 | 29052900 |
| 위험 문구(R) | 36/37/38 |
| 위험 표시 | Xi |
| 안전 문구(S) | 26-36-36/37/39 |
| F 고인화성물질 | 3 |
| 저장 분류 | 13 - Non Combustible Solids |
용도
용도
Good's buffers Tricine is used as an electrophoresis buffer and involved in the separation of low molecular weight proteins and peptides. It operates in the pH range between 7.4 and 8.8. It acts as an effective scavenger of hydroxyl radicals during the study of radiation-induced membrane damage. It is also used in resuspension of cell pellets. It is a zwitterionic amino acid commonly useful in the pH range of 7.4-8.8 and the pKa is 8.05 at 25°C. This buffer is also used as an eluent in asymmetrical flow field-flow fractionation (AFFFF).
일반 설명
Tricine and its salts are used as biological buffers to control the pH of body fluids in vitro and in vivo. It acts as a precursor for polymers, oxazolones (with carboxylic acids), and oxazolidines (with aldehydes). Trizma can serve as an alkalizing agent in treating acidosis of the blood.