5-브로모-4-클로로-3-인돌릴포스페이트p-톨루이딘염
5-브로모-4-클로로-3-인돌릴포스페이트p-톨루이딘염 · BCIP · BCIP P-TOLUIDINE SALT · BClP · BCIP-T · abs9176 · BCIP P-TOL · X-PHOS P-TOL
식별자 및 물리적 특성
| CAS No. | 6578-06-9 |
|---|---|
| 분자식 | C15H15BrClN2O4P |
| 평균 분자량 | 433.62 g/mol |
| 단동위원소 질량 | 431.96413337 g/mol |
| SMILES | Cc1ccc(N)cc1.O=P(O)(O)Oc1c[nH]c2ccc(Br)c(Cl)c12 |
| InChIKey | QEIFSLUFHRCVQL-UHFFFAOYSA-N |
| PubChem CID | 81059 |
| EC No. | 229-506-1 |
| DTXSID | DTXSID0064412 |
| 현지명 | 5-브로모-4-클로로-3-인돌릴포스페이트p-톨루이딘염 |
화학 및 물리적 성질
| 극성 표면적 | 109 |
|---|---|
| 수소 결합 공여자 수 | 4 |
| 수소 결합 수용자 수 | 5 |
| 회전 가능 결합 수 | 2 |
| 무거운 원자 수 | 24 |
| 형식 전하 | 0 |
| 복잡도 | 377 |
| 물질 분류 | Nitrogen Compounds -> Indoles |
| Physical Description | Solid[Sigma-Aldrich MSDS] |
| 녹는점 | 194-195℃ |
| 저장 조건 | -20°C |
| 용해도 | DMF: 20mg/mL |
| 물리적 상태 | 플레이크 크리스탈 |
| 색상 | White to Orange to Green |
| InChI | InChI=1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)141-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14)2-5H,8H2,1H3 |
| InChIKey | QEIFSLUFHRCVQL-UHFFFAOYSA-N |
| SMILES | C12C(Cl)=C(C=CC=1NC=C2OP(=O)(O)O)Br.C1(C)C=CC(N)=CC=1 |
| EPA | 1H-Indol-3-ol, 5-bromo-4-chloro-, dihydrogen phosphate (ester), compd. with 4-methylbenzenamine (1:1) (6578-06-9) |
화학적 성질
White to light brown powder
안전 및 규제
| GHS 분류 | WarningH302 (52.2%): Harmful if swallowed [Warning Acute toxicity, oral]P261, P264, P264+P265, P270, P271, P280, P301+P317, P302+P352, P304+P340, P305+P351+P338, P317, P319, P321, P330, P332+P317, P337+P317, P362+P364, P403+P233, P405, and P501 (click each P-code to see the statement)Aggregated GHS information provided per 23 reports by companies from 12 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies. |
|---|---|
| 유해성 요약 | A skin and strong eye irritant[Sigma-Aldrich MSDS] See p-Toluidine and 5-Bromo-4-chloro-3-indoxyl phosphate. |
| Hazard Classes and Categories | Acute Tox. 4 (52.2%) |
| 수질 위해 등급(WGK) | 3 |
| TSCA 등재 상태 | TSCA listed |
| 운송 번호(UN) | 2811 |
| 관세 코드 | 29339190 |
| 위험 문구(R) | 36/37/38-40 |
| 위험 표시 | Xi |
| 안전 문구(S) | 26 |
| 포장분류 | III |
| 위험 등급 | 6.1 |
| F 고인화성물질 | 3-8-10 |
| 저장 분류 | 11 - Combustible Solids |
용도
용도
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
일반 설명
BCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected.