Hepps
EPPS · 4-(2-HYDROXYETHYL)-1-PIPERAZINEPROPANESULFONIC ACID · HEPE · aEPPS · HEPPS · EPPS,99% · HEPPS(EPPS) · HEPPS Buffer
식별자 및 물리적 특성
| CAS No. | 16052-06-5 |
|---|---|
| 분자식 | C9H20N2O4S |
| 평균 분자량 | 252.33 g/mol |
| 단동위원소 질량 | 252.11437812 g/mol |
| SMILES | O=S(=O)(O)CCCN1CCN(CCO)CC1 |
| InChIKey | OWXMKDGYPWMGEB-UHFFFAOYSA-N |
| PubChem CID | 85255 |
| ChEBI | CHEBI:42298 |
| EC No. | 240-198-8 |
| DTXSID | DTXSID3066003 |
화학 및 물리적 성질
| XLogP | -3.6 |
|---|---|
| 극성 표면적 | 89.5 |
| 수소 결합 공여자 수 | 2 |
| 수소 결합 수용자 수 | 6 |
| 회전 가능 결합 수 | 6 |
| 무거운 원자 수 | 16 |
| 형식 전하 | 0 |
| 복잡도 | 280 |
| 물질 분류 | Nitrogen Compounds -> Piperazines |
| Physical Description | Beige powder[Sigma-Aldrich MSDS] |
| 녹는점 | 237-239 °C(lit.) |
| 밀도 | 1.2684 (rough estimate) |
| 굴절률 | 1.6800 (estimate) |
| 저장 조건 | Sealed in dry,Room Temperature |
| 용해도 | H2O: 1 M at 20 °C, 투명, 무색 |
| 물리적 상태 | 분말/고체 |
| 색상 | 하얀색 |
| pH | 5.0-6.5 (25℃, 0.1M in H2O) |
| pKa(산도 계수) | 8.0(at 25℃) |
| 용해도 | 물에 용해됩니다. 25.2g/L(20°C) |
| BRN | 3957385 |
| 주요 응용 | 진단 검사 제조 |
| InChI | 1S/C9H20N2O4S/c12-8-7-11-5-3-10(4-6-11)2-1-9-16(13,14)15/h12H,1-9H2,(H,13,14,15) |
| InChIKey | OWXMKDGYPWMGEB-UHFFFAOYSA-N |
| SMILES | OCCN1CCN(CCCS(O)(=O)=O)CC1 |
| EPA | 1-Piperazinepropanesulfonic acid, 4-(2-hydroxyethyl)- (16052-06-5) |
| Absorption | ≤0.1 at 260 in H2O at 1M≤0.1 at 280 in H2O at 1M |
화학적 성질
white crystalline powder
안전 및 규제
| GHS 분류 | This chemical does not meet GHS hazard criteria for 94.2% (65 of 69) of all reports.Not ClassifiedAggregated GHS information provided per 69 reports by companies from 6 notifications to the ECHA C&L Inventory. |
|---|---|
| 규제 정보 | Chemical: 1-Piperazinepropanesulfonic acid, 4-(2-hydroxyethyl)-Status: Active Update: 09-09-2021 https://echa.europa.eu/registration-dossier/-/registered-dossier/327991-Piperazinepropanesulphonic acid, 4-(2-hydroxyethyl)-: Does not have an individual approval but may be used under an appropriate group standard |
| 유해성 요약 | Oral LD50 (quail) > 316 mg/kg[RTECS] An eye irritant[Sigma-Aldrich MSDS] See Piperazine and salts. See HEPES. |
| Hazard Classes and Categories | Not Classified |
| 수질 위해 등급(WGK) | 3 |
| TSCA 등재 상태 | TSCA listed |
| RTECS 번호 | TM2649130 |
| 독성 | quail,LD50,oral,> 316mg/kg (316mg/kg),Ecotoxicology and Environmental Safety. Vol. 6, Pg. 149, 1982. |
| 관세 코드 | 29335995 |
| 위험 문구(R) | 36/37/38-22 |
| 위험 표시 | Xi |
| 안전 문구(S) | 24/25-36-26 |
| 위험 등급 | IRRITANT |
| 저장 분류 | 11 - Combustible Solids |
용도
용도
HEPPS (EPPS) is a commonly used Good's buffer. It is often used as separator in ultrathin isoelectric focusing gels and enhances the resolution of phosphoglucomutase. It is used as a buffering agent in biology and biochemistry. HEPPS is a biological buffer with a useful pH range of 7.3-8.7 and a pKa=8.0 at 25°C. Research on mice with Alzheimer's disease-like amyloid beta plaques has shown that HEPPS can cause the plaques to break up, reversing some of the symptoms in the mice. HEPPS was reported to dissociate amyloid beta oligomers in patients' plasma samples enabling blood diagnosis of Alzheimer's disease.
일반 설명
HEPPS, or N-(2-Hydroxyethyl)piperazine-N′-(3-propanesulfonic acid), is a zwitterionic buffering agent commonly used in biochemical and molecular biology research. Its mechanism of action is rooted in its ability to maintain a stable pH environment, which is crucial for various enzymatic reactions and protein studies. HEPPS is especially favoured for its buffering capacity in the physiological pH range, typically between pH 6.8 and 8.2, making it ideal for biological assays and cell culture experiments. This compound is extensively utilized in preparing cell culture media, where maintaining a consistent pH is essential for cell viability and growth. Additionally, HEPPS is employed in biochemical assays, such as enzyme kinetics studies, where precise pH control is necessary to accurately measure enzymatic activity. Furthermore, HEPPS has found applications in molecular biology techniques, including nucleic acid purification, PCR (polymerase chain reaction), and protein analysis by SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). Its compatibility with various biological systems and stability under physiological conditions make HEPPS a valuable tool in research laboratories for maintaining optimal pH conditions and ensuring reliable experimental outcomes in various biochemical and molecular biology studies.