비신
비신 · N,N-비스(2-하이드록시에틸)글리신 · BICINE BUFFER · N,N-DI(2-HYDROXYETHYL)GLYCINE · N,N-BIS(2-HYDROXYETHYL)GLYCINE · BIClNE · BICINE · Bicirne
식별자 및 물리적 특성
| CAS No. | 150-25-4 |
|---|---|
| 분자식 | C6H13NO4 |
| 평균 분자량 | 163.17 g/mol |
| 단동위원소 질량 | 163.0844579 g/mol |
| SMILES | O=C(O)CN(CCO)CCO |
| InChIKey | FSVCELGFZIQNCK-UHFFFAOYSA-N |
| PubChem CID | 8761 |
| ChEMBL | CHEMBL1231251 |
| ChEBI | CHEBI:40957 |
| EC No. | 205-755-1 |
| DTXSID | DTXSID3041669 |
| 현지명 | 비신 |
화학 및 물리적 성질
| XLogP | -3.8 |
|---|---|
| 극성 표면적 | 81 |
| 수소 결합 공여자 수 | 3 |
| 수소 결합 수용자 수 | 5 |
| 회전 가능 결합 수 | 6 |
| 무거운 원자 수 | 11 |
| 형식 전하 | 0 |
| 복잡도 | 111 |
| 물질 분류 | Other Uses -> Biological Buffers |
| 의약품 | Pharmaceuticals -> Listed in ZINC15 |
| Physical Description | Solid[Merck Index] White crystalline powder[Alfa Aesar MSDS] |
| 녹는점 | 190 °C (dec.)(lit.) |
| 끓는 점 | 290.25°C (rough estimate) |
| 밀도 | 1.05 g/mL at 20 °C |
| 증기압 | 0.002-0.007Pa at 20-25℃ |
| 굴절률 | 1.4240 (estimate) |
| 저장 조건 | room temp |
| 용해도 | H2O: 1 M at 20 °C, 투명, 무색 |
| 물리적 상태 | 가루 |
| 색상 | 하얀색 |
| pH | 4.0-5.5 (25℃, 1M in H2O) |
| pKa(산도 계수) | 8.3(at 25℃) |
| 용해도 | 녹는 |
| Merck | 14,1201 |
| BRN | 1769362 |
| InChI | 1S/C6H13NO4/c8-3-1-7(2-4-9)5-6(10)11/h8-9H,1-5H2,(H,10,11) |
| InChIKey | FSVCELGFZIQNCK-UHFFFAOYSA-N |
| SMILES | OCCN(CCO)CC(O)=O |
| LogP(분배 계수) | 2.3 at 20℃ and pH7 |
| 표면장력 | 72.2mN/m at 1g/L and 20℃ |
| EPA | Bicine (150-25-4) |
| Absorption | ≤0.05 at 260 in H2O at 1M≤0.05 at 280 in H2O at 1M |
화학적 성질
Bicine is a white/clear crystalline powder that is somewhat soluble in water. It contains an amino group, carboxyl group, and two hydroxyl units in its structure, making it highly polar. It is often used as a biological buffer and chelating agent.
안전 및 규제
| GHS 분류 | This chemical does not meet GHS hazard criteria for 97.6% (124 of 127) of all reports.Not ClassifiedAggregated GHS information provided per 127 reports by companies from 4 notifications to the ECHA C&L Inventory. |
|---|---|
| 규제 정보 | Chemical: Glycine, N,N-bis(2-hydroxyethyl)-Status: Active Update: 23-06-2022 https://echa.europa.eu/registration-dossier/-/registered-dossier/23693Glycine, N,N-bis(2-hydroxyethyl)-: Does not have an individual approval but may be used under an appropriate group standard |
| 기타 안전 정보 | Evaluation - Chemicals that are unlikely to require further regulation to manage risks to environment |
| 유해성 요약 | Causes changes in tubules (including acute renal failure and necrosis) in intraperitoneal lethal-dose studies of mice (LD50 = 1,540 mg/kg)[RTECS] May cause irritation[Alfa Aesar MSDS] See Glycine. |
| Hazard Classes and Categories | Not Classified |
| 유해 작용 | Nephrotoxin - The chemical is potentially toxic to the kidneys in the occupational setting. |
| 수질 위해 등급(WGK) | 3 |
| TSCA 등재 상태 | TSCA listed |
| RTECS 번호 | MB9700000 |
| 독성 | mouse,LD50,intraperitoneal,1540mg/kg (1540mg/kg),KIDNEY, URETER, AND BLADDER: "CHANGES IN TUBULES (INCLUDING ACUTE RENAL FAILURE, ACUTE TUBULAR NECROSIS)",Revue d'Epidemiologie, Medecine Sociale et Sante Publique. Vol. 10, Pg. 391, 1962. |
| 관세 코드 | 29225000 |
| 위험 문구(R) | 20/21/22-36/37/38 |
| 위험 표시 | Xn |
| 안전 문구(S) | 24/25-36-26 |
| 기존화학 물질 | 2013-3-5652 |
| F 고인화성물질 | 3-10 |
| 저장 분류 | 11 - Combustible Solids |
용도
개요
Bicine is an amino acid that results from amine degradation due to the presence of oxygen and/or sulfur dioxide. The amine solvents that undergo this degradation are MDEA, DEA, TEA, and mixed amine solvents containing any of these amines. As a zwitterionic amino acid buffer agent, bicine is active in the pH 7.6-9.0 range (pKa of 8.26 at 25°C). Bicine is a recommended buffer for low-temperature biochemical work. It is used for the preparation of stable substrate solution for serum guanase determination. It can be used as a nitrogenous strength enhancer to regulate cement hydration and improve cement properties. Studies have found that bicine significantly changes the morphology of portlandite crystals from a parallel-stacked lamellar shape to a distorted one and also decreases the pore size of the hardened cement paste at an early age[1]. In particular, bicine could promote the rapid formation of β-sheet-rich amyloid-β fibrils[2].
용도
A zwitterionic amino acid buffer agent used for low temperature work. The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published. Bicine has been utilized in peptide and protein crystallization. A kinetic study of a quaternary transition-state analogue complex of creatine kinase used bicine in the reaction buffer. A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described.