4,4'-((4-Iminocyclohexa-2,5-dien-1-ylidene)methylene)bis(N,N-dimethylaniline) monohydrochloride

Methyl violet 2B is a hydrochloride obtained by combining Methyl violet 2B free base with one equivalent of hydrochloric acid. Used in Gram's stain for the demonstration and primary classification of bacteria as well as to demonstrate amyloid. It has a role as a mutagen, an antineoplastic agent, a fluorochrome, an EC 2.7.10.2 (non-specific protein-tyrosine kinase) inhibitor and a histological dye. It contains a Methyl violet 2B(1+).