트리스(하이드록시메틸)아미노메탄 수화염화물
트리스(하이드록시메틸)아미노메탄수화염화물 · 2-아미노-2-(히드록시메틸)-1,3-프로판디올염산염 · TRIS-HCL · Tris-HCI · TRIS(HYDROXYMETHYL)AMINOMETHANE HYDROCHLORIDE · TRIZMA HYDROCHLORIDE · TRIS-HCL BUFFER · Tris-HC
식별자 및 물리적 특성
| CAS No. | 1185-53-1 |
|---|---|
| 분자식 | C4H11NO3.ClH |
| 평균 분자량 | 157.59 g/mol |
| 단동위원소 질량 | 157.05057092 g/mol |
| SMILES | Cl.NC(CO)(CO)CO |
| InChIKey | QKNYBSVHEMOAJP-UHFFFAOYSA-N |
| PubChem CID | 93573 |
| ChEMBL | CHEMBL3183206 |
| EC No. | 214-684-5 |
| DTXSID | DTXSID4044373 |
| 현지명 | 트리스(하이드록시메틸)아미노메탄 수화염화물 |
화학 및 물리적 성질
| 극성 표면적 | 86.7 |
|---|---|
| 수소 결합 공여자 수 | 5 |
| 수소 결합 수용자 수 | 4 |
| 회전 가능 결합 수 | 3 |
| 무거운 원자 수 | 9 |
| 형식 전하 | 0 |
| 복잡도 | 54 |
| 물질 분류 | Nitrogen Compounds -> Amino Alcohols, Other |
| Physical Description | Dry PowderLiquidOther SolidHygroscopic solid[Sigma-Aldrich MSDS] |
| 녹는점 | 150-152 °C |
| 끓는 점 | 225℃[at 101 325 Pa] |
| 밀도 | 1.05 g/mL at 20 °C |
| 증기압 | 0Pa at 20℃ |
| 저장 조건 | room temp |
| 용해도 | H2O: 4 M at 20 °C, 투명, 무색 |
| 물리적 상태 | 수정 같은 |
| 색상 | 투명한 무색(H2O에서 40 %(w/w)) 용액 |
| pH | 2.5-4.0 (25℃, 4M in H2O) |
| pKa(산도 계수) | 8.1(at 25℃) |
| 생물학적 소스 | synthetic |
| 용해도 | 녹는 |
| BRN | 3675235 |
| 안정성 | 안정적인. 강한 산화제, 염기, 습기와 호환되지 않습니다. |
| 화장품 원료 기능 | 항균 |
| InChI | 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H21H |
| InChIKey | QKNYBSVHEMOAJP-UHFFFAOYSA-N |
| SMILES | Cl.NC(CO)(CO)CO |
| LogP(분배 계수) | -3.6 at 20℃ |
| EPA | 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride (1185-53-1) |
| Absorption | ≤0.05 at 290nm at 40% |
화학적 성질
TRIS hydrochloride is a white crystalline powder that has a pKa of 8.08 at 25°C and pH range of 7.0 to 9.0 that coincides with the physiological pH of most living organisms because of which it is commonly used as a component of buffer solutions in biology, biochemistry and molecular biology applications. Tris salts are also used for crystallization of proteins at various pH values. Neither Tris base nor Tris hydrochloride by itself provide adequate buffering capacity. Generally the two need to be mixed together to provide a buffer with pH between 7 and 9 to provide adequate buffering.
안전 및 규제
| GHS 분류 | This chemical does not meet GHS hazard criteria for 57.2% (103 of 180) of all reports.WarningH315 (42.2%): Causes skin irritation [Warning Skin corrosion/irritation]P261, P264, P264+P265, P271, P280, P302+P352, P304+P340, P305+P351+P338, P319, P321, P332+P317, P337+P317, P362+P364, P403+P233, P405, and P501 (click each P-code to see the statement)Aggregated GHS information provided per 180 reports by companies from 16 notifications to the ECHA C&L Inventory. Each notification may be associated with multiple companies. |
|---|---|
| 규제 정보 | Chemical: 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride (1:1) is listed on the EPA's Chemical Data Reporting (CDR) system. Manufacturers and importers of 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride (1:1) are required to report information about their production and use of this chemical to the EPA under the Toxic Substances Control Act (TSCA). (40 eCFR Part 711)Status: Active Update: 09-12-2022 https://echa.europa.eu/registration-dossier/-/registered-dossier/193062-amino-2-(hydroxymethyl)propane-1,3-diol: Does not have an individual approval but may be used under an appropriate group standard |
| 유해성 요약 | A mild eye irritant in rabbitsNot a sensitizer in experimental animals[Sigma-Aldrich MSDS] See Tromethamine. |
| Hazard Classes and Categories | Skin Irrit. 2 (42.2%) |
| Protective Action Criteria (PAC) | 18 [mg/m3]190 [mg/m3]1200 [mg/m3] |
| 수질 위해 등급(WGK) | 3 |
| TSCA 등재 상태 | TSCA listed |
| RTECS 번호 | TY2900000 |
| 관세 코드 | 29221980 |
| 위험 문구(R) | 36/37/38 |
| 위험 표시 | Xi |
| 안전 문구(S) | 26-36 |
| 기존화학 물질 | KE-34819 |
| F 고인화성물질 | 3 |
| 저장 분류 | 11 - Combustible Solids |
용도
개요
Abbreviated as Tris HCl (TRIS hydrochloride), Tris (hydroxymethyl) aminomethane hydrochloride is an organic compound commonly utilized in buffer solutions such as TAE or TBE for electrophoresis gels. It boasts high solubility in water and offers utility within the pH range of 7.0-9.0.
용도
Tris Hydrochloride is a buffering medium for electrophoresis, molecular biology, and cell culture applications. It may be combined with Tris Base to simplify Tris buffer preparation. Tris Hydrochloride has been incorporated in coelomic fluid and in Cortland medium to store unfertilized rainbow trout eggs. In immunohistochemistry, Tris Hydrochloride has also been used with urea as a method for antigen retrieval.
일반 설명
TRIS hydrochloride is mainly required for preparation of buffer at physiological range of 7.3 to 7.5. The prepared buffer is compatible with biological fluids. It is of importance to laboratories as a standard pH solution.
제조 및 합성 방법
제조 방법
The preparation of TRIS hydrochloride is as follows:After reacting 2-amino-1,3-propanediol (2.50 g, 27.44 mmol) and formic acid (1.26 g, 27.44 mmol) in 50 ml of water at room temperature for 3 hours, water was distilled off under reduced pressure, A liquid was obtained. By washing the obtained liquid,An ammonium formate salt (3.76 g, 27.44 mmol) was obtained as a colorless transparent liquid.