Huagongshe화공사 화학 정보 인쇄 보고서 · 연구 참고용
Sodium Chloride → Leukotriene B4
참여 물질
| 역할 | 이름 | HCID | 투입량 |
|---|---|---|---|
| 반응물 | Sodium Chloride | HCID 5234 | — |
| 반응물 | Ethylenediaminetetraacetic Acid | HCID 6049 | — |
| 반응물 | Leukotriene A4 | HCID 5280383 | — |
| 반응물 | methyl 4-[(2S,3S)-3-[(1E,3E,5Z,8Z)-tetradeca-1,3,5,8-tetraenyl]oxiran-2-yl]butanoate | HCID 6478221 | — |
| 용매 | Potassium Phosphate, Tribasic | HCID 62657 | — |
| 생성물 | Leukotriene B4 | HCID 5280492 | — |
반응 단계
The LTA4 substrate was freshly prepared by alkaline hydrolysis of LTA4 methyl ester (Cayman Chemical Co., USA). After incubating for 10 minutes in the presence of LTA4 (1 μM in 50 mM Tris-HCl, 0.15 M NaCl, 0.5% BSA, pH 7.4), the reaction is stopped by diluting 1/20 in 0.1 M potassium phosphate buffer containing 1.5 mM NaN2, 0.4 M NaCl, 1 mM EDTA, 0.1% BSA, pH 7.4, −4° C.
출처 및 근거
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 특허 번호 | US07094773B2 |
| 출처 데이터셋 | uspto-grants-2006_08 |
| ORD ID | ord-2c4f74d36a3e4147b27538559e142388 |
| 출처 메모 | uspto-grants-2006_08 |