tert-Butyl methyl ether → HCID 9793812, 화합물

반응식

[CH:1]([CH:4]([CH:8]([CH3:10])[CH3:9])[C:5]([NH2:7])=[O:6])([CH3:3])C.[CH3:11]OC(C)(C)C>>[CH2:1]([C@@H:4]([CH:8]([CH3:9])[CH3:10])[C:5]([NH2:7])=[O:6])[CH2:3][CH3:11]

참가 물질

반응물 2

HCID 15413tert-Butyl methyl ether

HCID 228084Diisopropylacetamide

생성물 1

HCID 9793812HCID 9793812, 화합물

실험 절차

Assay of plasma samples: to test tubes containing 2 μg of the internal standard (diisopropyl acetamide), 0.5 ml of plasma (thawed at room temperature) and 5 ml of tert-butyl methyl ether (TBME) was added. The test tubes were vigorously vortexed for 30 sec and centrifuged at 3000 g for 10 minutes. The organic phase was separated and dried under reduced pressure using a vortex evaporator. Samples were then reconstituted with 150 μl of chloroform and dried under reduced pressure without vortexing. The sample was again reconstituted with 30 μl of chloroform, of which 2 μl were injected into the GC apparatus.

후처리

additionwas added
waitcentrifuged at 3000 g for 10 minutes
customThe organic phase was separated
customdried under reduced pressure
customa vortex evaporator
customdried under reduced pressure without vortexing

출처 및 근거

ORD 기록: ord-19039cf30e564ed1a92aacb0a5922940
출처 데이터셋: uspto-grants-2005_11
DOI: 10.6084/m9.figshare.5104873.v1
특허 번호: US06969732B2

반응 노트

불러오는 중…