Huagongshe화공사 화학 정보 인쇄 보고서 · 연구 참고용
Sucrose → Ethylenediaminetetraacetic Acid
참여 물질
| 역할 | 이름 | HCID | 투입량 |
|---|---|---|---|
| 반응물 | Sucrose | HCID 5988 | — |
| 반응물 | Bromophenol Blue | HCID 8272 | — |
| 반응물 | Ethidium Bromide | HCID 14710 | — |
| 반응물 | Xylene cyanol | HCID 130146 | — |
| 반응물 | 2-Amino-2-(hydroxymethyl)propane-1,3-diol;2-(2-(bis(carboxymethyl)amino)ethyl-(carboxymethyl)amino)acetic acid;boric acid | HCID 21257725 | — |
| 용매 | Water | HCID 962 | — |
| 생성물 | Ethylenediaminetetraacetic Acid | HCID 6049 | — |
| 생성물 | Boric Acid | HCID 7628 | — |
반응 단계
The PCR reaction was monitored by agarose gel electrophoresis as follows: A 1.5% agarose (FMC Bioproducts) electrophoresis gel in tris-borate EDTA (“TBE”) 1× buffer (89 mM Tris-borate (Fisher Biotech), 89 mM boric acid (Mallinckrodt) and 2 mM EDTA (Mallinckrodt)) was prepared. Five to ten microliters of PCR products were loaded onto the gel with 1 μl dye 10× (0.5% bromophenol blue (Sigma), 0.5% Xylene cyanol (Gibco-BRL), 40% (w/v) sucrose (IBI) in water). Electrophoresis was run in TBE 1× at 120 V for 45 minutes. The gel was stained with ethidium bromide (10 μg/ml, Sigma) for 5 minutes. PCR products were visualized with ultraviolet light.
출처 및 근거
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 특허 번호 | US06242182B1 |
| 출처 데이터셋 | uspto-grants-2001_06 |
| ORD ID | ord-aeeabda2c02645fc96178ef2414a9773 |
| 출처 메모 | uspto-grants-2001_06 |