Phosphate → aldehydo-D-Xylose

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Xylose (100 gm) was dissolved in distilled water to provide a concentration of 60% w/v. The solution was placed in a reaction vessel to which was added CoCl2 and MgCl2 to provide a concentration of Co++ =10-4M and Mg++ =10-3M. The pH of the solution was adjusted to 7.5 by the addition of a phosphate buffer. To the resulting solution was then added 5 gm of glucose isomerase extracted from Streptomyces and the temperature was maintained at 60° C. overnight (18 hours). The solution was then added to a fermentation medium (DIFCO 8.5 gm/l yeast extract, NH4Cl1.3 gm/l, MgSO4 ·7 H2O·11 gm/l, and CaCl2 .06 gm/l) to yield an initial fermentation substrate concentration of 78 gm/l xylose and 20 gm/l xylulose. The resulting pH was 5.5.

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