2-Trans,6-trans,10-trans-geranylgeranyl diphosphate(3-) → Stemod-13(17)-ene

HRID 1172036노트

반응식

HRID 1172036 반응식
O([CH2:10]/[CH:11]=[C:12](/[CH2:14][CH2:15]/[CH:16]=[C:17](\[CH3:29])/[CH2:18][CH2:19]/[CH:20]=[C:21](/[CH2:23][CH2:24][CH:25]=[C:26]([CH3:28])[CH3:27])\[CH3:22])\[CH3:13])P(OP([O-])([O-])=O)(=O)[O-].CC1[C@H]2C[C@]3(CC2)[C@@H](CC[C@H]2C(C)(C)CCC[C@@]23C)C=1>CCCCC>[CH3:22][C@@:21]12[C@:16]34[CH2:15][C@H:14]([CH2:29][C@@H:17]3[CH2:18][CH2:19][C@H:20]1[C:26]([CH3:28])([CH3:27])[CH2:25][CH2:24][CH2:23]2)[C:12](=[CH2:13])[CH2:11][CH2:10]4

참가 물질

4

반응물 2

HCID 54971052-Trans,6-trans,10-trans-geranylgeranyl diphosphate(3-)HCID 15161496Stemar-13-ene

생성물 1

HCID 24798689Stemod-13(17)-ene수율 85%

시약·촉매·용매 1

HCID 8003Pentane용매

실험 절차

Chemicals. The preparations of (E,E,E)-geranylgeranyl diphosphate (GGPP), and ent- and syn-copalyl diphosphate (CPP), and syn-stemarene have been previously described. (Mohan et al. 1996). Stemodene standards were derived from a 1.5:1 mixture (ca. 5 mg) of synthetic racemic exo- and endo-stemodenes, kindly provided by Jim White. (White et al. 1994). This was fractionated by flash chromatography on a silica gel column (1 cm×10 cm) using pentane and the later eluting fractions (enriched for the exo isomer) were pooled and concentrated under nitrogen to yield ˜2 mg exo-stemodene (˜85% purity by GC analysis). Unless otherwise noted, chemicals were purchased from Fisher Scientific (Loughborough, Leicestershire, UK) and molecular biology reagents from Invitrogen (Carlsbad, Calif., USA).

후처리

  1. customkindly provided by Jim White
  2. washthe later eluting fractions (
  3. concentrationconcentrated under nitrogen

출처 및 근거

ORD 기록
ord-8176d06d5b934868a41922c3b6d98eb9
출처 데이터셋
uspto-grants-2010_11
특허 번호
US07833748B1

반응 노트

불러오는 중…