Acetate → ASIALOGANGLIOSIDE-GM1

HRID 1197403노트

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HRID 1197403 반응식
[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][C:18]([NH:20][C@H:21]([C@H:91]([OH:107])/[CH:92]=[CH:93]/[CH2:94][CH2:95][CH2:96][CH2:97][CH2:98][CH2:99][CH2:100][CH2:101][CH2:102][CH2:103][CH2:104][CH2:105][CH3:106])[CH2:22][O:23][C@@H:24]1[O:29][C@H:28]([CH2:30][OH:31])[C@@H:27]([O:32][C@@H:33]2[O:38][C@H:37]([CH2:39][OH:40])[C@H:36]([O:41][C@@H:42]3[O:47][C@H:46]([CH2:48][OH:49])[C@H:45]([OH:50])[C@H:44]([O:51][C@@H:52]4[O:57][C@H:56]([CH2:58][OH:59])[C@H:55]([OH:60])[C@H:54]([OH:61])[C@H:53]4[OH:62])[C@H:43]3[NH:63][C:64]([CH3:66])=[O:65])[C@H:35]([O:67][C@@]3(C(O)=O)O[C@@H]([C@H](O)[C@H](O)CO)[C@H](NC(C)=O)[C@@H](O)C3)[C@H:34]2[OH:88])[C@H:26]([OH:89])[C@H:25]1[OH:90])=[O:19].C([O-])(=O)C>O>[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][C:18]([NH:20][CH:21]([CH:91]([OH:107])/[CH:92]=[CH:93]/[CH2:94][CH2:95][CH2:96][CH2:97][CH2:98][CH2:99][CH2:100][CH2:101][CH2:102][CH2:103][CH2:104][CH2:105][CH3:106])[CH2:22][O:23][CH:24]1[O:29][C@H:28]([CH2:30][OH:31])[C@@H:27]([O:32][C@@H:33]2[O:38][C@H:37]([CH2:39][OH:40])[C@H:36]([O:41][C@@H:42]3[O:47][C@H:46]([CH2:48][OH:49])[C@H:45]([OH:50])[C@H:44]([O:51][C@@H:52]4[O:57][C@H:56]([CH2:58][OH:59])[C@H:55]([OH:60])[C@H:54]([OH:61])[C@H:53]4[OH:62])[C@H:43]3[NH:63][C:64]([CH3:66])=[O:65])[C@H:35]([OH:67])[C@H:34]2[OH:88])[C@H:26]([OH:89])[C@H:25]1[OH:90])=[O:19]

참가 물질

4

반응물 2

HCID 175AcetateHCID 9963963Ganglioside GM1

생성물 1

HCID 6450363ASIALOGANGLIOSIDE-GM1

시약·촉매·용매 1

HCID 962Water용매

실험 절차

A 10 ml quantity of 0.05% ganglioside GM1 solution, 10 ml of 40 mM acetate buffer (pH 5.0) and 10 ml of sterilized water were mixed together, and to the mixture was added 10 ml of aqueous Isozyme L (3 U/ml), followed by reaction at 37° C. for 8 hours. The reaction mixture was subjected to purification in the same manner as in Example 1, giving about 2.6 mg of asialo GM1 as a white powder. The production of asialo GM1 was confirmed in the same manner as in Example 1.

후처리

  1. additionto the mixture was added 10 ml of aqueous Isozyme L (3 U/ml)
  2. customfollowed by reaction at 37° C. for 8 hours
  3. customThe reaction mixture was subjected to purification in the same manner as in Example 1

출처 및 근거

ORD 기록
ord-8521938072454751abdfcfe23566ef80
출처 데이터셋
uspto-grants-1994_01
특허 번호
US05275939

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