Hydrogen Peroxide → H-Gly-Gly-Phe-Met(O2)-Thr-Ser-Glu-Lys-Ser-Gln-Thr-Pro-Leu-Val-Thr-Leu-OH

HRID 127610

반응식

HRID 127610 반응식
[NH2:1][CH2:2][C:3]([NH:5][CH2:6][C:7]([NH:9][C@H:10]([C:18]([NH:20][C@H:21]([C:26]([NH:28][C@H:29]([C:33]([NH:35][C@H:36]([C:39]([NH:41][C@H:42]([C:48]([NH:50][C@H:51]([C:57]([NH:59][C@H:60]([C:63]([NH:65][C@H:66]([C:72]([NH:74][C@H:75]([C:79]([N:81]1[CH2:118][CH2:117][CH2:116][C@H:82]1[C:83]([NH:85][C@H:86]([C:91]([NH:93][C@H:94]([C:98]([NH:100][C@H:101]([C:105]([NH:107][C@H:108]([C:113]([OH:115])=[O:114])[CH2:109][CH:110]([CH3:112])[CH3:111])=[O:106])[C@@H:102]([CH3:104])[OH:103])=[O:99])[CH:95]([CH3:97])[CH3:96])=[O:92])[CH2:87][CH:88]([CH3:90])[CH3:89])=[O:84])=[O:80])[C@@H:76]([CH3:78])[OH:77])=[O:73])[CH2:67][CH2:68][C:69](=[O:71])[NH2:70])=[O:64])[CH2:61][OH:62])=[O:58])[CH2:52][CH2:53][CH2:54][CH2:55][NH2:56])=[O:49])[CH2:43][CH2:44][C:45](=[O:47])[OH:46])=[O:40])[CH2:37][OH:38])=[O:34])[C@@H:30]([CH3:32])[OH:31])=[O:27])[CH2:22][CH2:23][S:24][CH3:25])=[O:19])[CH2:11][C:12]1[CH:17]=[CH:16][CH:15]=[CH:14][CH:13]=1)=[O:8])=[O:4].[OH2:119].[OH:120]O.C(O)(C)(C)C.O>C([O-])(=O)C.[NH4+].[NH4+].[O-][Mo]([O-])(=O)=O>[NH2:1][CH2:2][C:3]([NH:5][CH2:6][C:7]([NH:9][C@H:10]([C:18]([NH:20][C@H:21]([C:26]([NH:28][C@H:29]([C:33]([NH:35][C@H:36]([C:39]([NH:41][C@H:42]([C:48]([NH:50][C@H:51]([C:57]([NH:59][C@H:60]([C:63]([NH:65][C@H:66]([C:72]([NH:74][C@H:75]([C:79]([N:81]1[CH2:118][CH2:117][CH2:116][C@H:82]1[C:83]([NH:85][C@H:86]([C:91]([NH:93][C@H:94]([C:98]([NH:100][C@H:101]([C:105]([NH:107][C@H:108]([C:113]([OH:115])=[O:114])[CH2:109][CH:110]([CH3:112])[CH3:111])=[O:106])[C@@H:102]([CH3:104])[OH:103])=[O:99])[CH:95]([CH3:96])[CH3:97])=[O:92])[CH2:87][CH:88]([CH3:90])[CH3:89])=[O:84])=[O:80])[C@@H:76]([CH3:78])[OH:77])=[O:73])[CH2:67][CH2:68][C:69](=[O:71])[NH2:70])=[O:64])[CH2:61][OH:62])=[O:58])[CH2:52][CH2:53][CH2:54][CH2:55][NH2:56])=[O:49])[CH2:43][CH2:44][C:45](=[O:46])[OH:47])=[O:40])[CH2:37][OH:38])=[O:34])[C@@H:30]([CH3:32])[OH:31])=[O:27])[CH2:22][CH2:23][S:24]([CH3:25])(=[O:120])=[O:119])=[O:19])[CH2:11][C:12]1[CH:17]=[CH:16][CH:15]=[CH:14][CH:13]=1)=[O:8])=[O:4]

참가 물질

7

반응물 4

HCID 784Hydrogen PeroxideHCID 962WaterHCID 3035192gamma-endorphin, des-Tyr(1)-HCID 714019492-Methylpropan-2-ol--water (1/1)

생성물 1

HCID 131712124H-Gly-Gly-Phe-Met(O2)-Thr-Ser-Glu-Lys-Ser-Gln-Thr-Pro-Leu-Val-Thr-Leu-OH

시약·촉매·용매 2

HCID 61578Diammonium molybdate촉매HCID 175Acetate용매

실험 절차

200 mg of the peptide obtained in Example I is introduced into 5 ml water after which 0.025 ml 0.5 M ammonium molybdate, 0.125 ml HClO4 and 0.075 ml 30% hydrogen peroxide are added. The mixture is stirred for about 4 hours at room temperature, after which 5 ml tert. butanol/water (1:1) and an ion exchange resin in acetate form are added. After stirring for about 30 minutes, the ion exchange resin is separated by filtration and the filtrate is evaporated to dryness.

후처리

  1. additionare added
  2. stirringAfter stirring for about 30 minutes
  3. customthe ion exchange resin is separated by filtration
  4. customthe filtrate is evaporated to dryness

출처 및 근거

ORD 기록
ord-2a341be9cf174b93bee52dc29bc0c541
출처 데이터셋
uspto-grants-1981_03
특허 번호
US04256736

연결된 노트

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