N-Methylpropanamide → Propanedioic acid monopropyl ester

HRID 1282411

반응식

HRID 1282411 반응식
C([O:4][C:5](=[O:9])[CH2:6][C:7]#N)CC.C[O:11]C(=O)CCC(O)=O.CN[C:21](=[O:24])[CH2:22][CH3:23]>P([O-])([O-])([O-])=O.[K+].[K+].[K+]>[CH2:21]([O:24][C:7](=[O:11])[CH2:6][C:5]([OH:4])=[O:9])[CH2:22][CH3:23]

참가 물질

5

반응물 3

HCID 14470N-MethylpropanamideHCID 77487monoMethyl succinateHCID 84444Propyl cyanoacetate2회

생성물 1

HCID 9920416Propanedioic acid monopropyl ester

시약·촉매·용매 1

HCID 62657Potassium Phosphate, Tribasic용매2회

반응 조건

온도
25 °C

실험 절차

Into a 10-mL volumetric flask was added 0.1271 g (1.00 mmol, 0.100 M) of cyanoacetic acid propyl ester, 9.689 mL of potassium phosphate buffer (50 mM, pH 7.0), and 0.20 mL of a 50 wt % cell suspension (0.10 g wet cell weight, 0.024 g dry cell weight) of Acidovorax facilis 72W (ATCC 55746) cells in 0.35 M potassium phosphate buffer (pH 7.0, previously heat-treated at 50° C. for 1h), and the resulting 10 mL suspension stirred at 25° C. Samples (0.180 mL) were mixed with 0.020 mL of 0.750 M N-methylpropionamide (HPLC external standard solution). The resulting solution was analyzed by HPLC. After 5 h, the conversion of cyanoacetic acid propyl ester was complete, and the yield (determined by HPLC and NMR) of propanedioic acid monopropyl ester was 100%, with no production of cyanoacetic acid or propanedioic acid.

후처리

  1. additionInto a 10-mL volumetric flask was added

출처 및 근거

ORD 기록
ord-e68732778b9a4090a3f420438d37b877
출처 데이터셋
uspto-grants-2002_09
특허 번호
US06455730B1

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