참여 물질
| 역할 | 이름 | HCID | 투입량 |
|---|
| 반응물 | Sodium Hydroxide | HCID 14798 | — |
| 반응물 | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| 반응물 | Biotin | HCID 171548 | — |
| 반응물 | Monobasic potassium phosphate | HCID 516951 | — |
| 반응물 | Ammonium Sulfate | HCID 6097028 | — |
| 반응물 | Thiaminhydrochloride | HCID 67590811 | — |
| 촉매 | Platinum | HCID 23939 | — |
| 생성물 | L-Lysine | HCID 5962 | — |
반응 단계
A medium containing 45 g/L of glucose, 15 g/L of molasses, 2 g/L (as nitrogen) of soybean protein hydrolysate, 2 g/L of KH2PO4, 5.6 g/L of NaOH, 10 g/L of ammonium sulfate, 0.8 g/L of MgSO4.7H2O, 20 mg/L of FeSO4.7H2O, 20 mg/L of MnSO4.4H2O, 0.8 mg/L of thiamin hydrochloride and 0.2 mg/L of biotin (pH 6.0) was introduced into 1-L volume small glass fermentation tank in a volume of 300 mL and sterilized by heating at 120° C. for 20 minutes. After the fermentation tank was cooled to 31.5° C., 5 platinum loops of Brevibacterium lactofermentum ATCC 31269 (refer to U.S. Pat. No. 4,066,501) preliminarily grown on an LB plate for 24 hours was inoculated to the medium and cultured at 31.5° C. and pH 7.0 for 30 hours with sufficient aeration and stirring.
출처 및 근거
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| 특허 번호 | US07883878B2 |
|---|
| 출처 데이터셋 | uspto-grants-2011_02 |
|---|
| ORD ID | ord-29426e88174549fe882c745e35c21a93 |
|---|
| 출처 메모 | uspto-grants-2011_02 |
|---|
