Br[CH2:2][C:3]([C:5]1[C:6](=[O:35])[N:7]([CH3:34])[C:8]2[C:13]([C:14]=1[NH:15][C:16](=[O:18])[CH3:17])=[CH:12][C:11]([C:19]1[CH:24]=[CH:23][C:22]([Cl:25])=[CH:21][CH:20]=1)=[C:10]([C:26]1[CH:31]=[CH:30][C:29]([Cl:32])=[CH:28][C:27]=1[Cl:33])[N:9]=2)=[O:4].CC([O-])=O.[Na+]>CN(C=O)C.O.CCOC(C)=O>[C:16]([N:15]1[C:14]2[C:13]3[CH:12]=[C:11]([C:19]4[CH:24]=[CH:23][C:22]([Cl:25])=[CH:21][CH:20]=4)[C:10]([C:26]4[CH:31]=[CH:30][C:29]([Cl:32])=[CH:28][C:27]=4[Cl:33])=[N:9][C:8]=3[N:7]([CH3:34])[C:6](=[O:35])[C:5]=2[C:3]([OH:4])=[CH:2]1)(=[O:18])[CH3:17]To the product of Step A (25 mg) was added NaOAc (60 mg) in DMF (2.5 mL) and water (0.3 mL) at room temperature. The reaction stirred overnight and was diluted with EtOAc, washed with saturated aqueous NaHCO3 and brine. The concentrated residue was purified by flash chromatography on silica gel with a gradient elution of 0-100% EtOAc in hexane affording the title compound. HPLC/MS: 512.0 (M+1), 514.0 (M+3); Rt=4.18 min.
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