Triethylamine → 4-(4-Aminoquinazolin-7-ylmethyl)-3-oxopiperazine-1-carboxylic acid (5-chloro-thiophen-2-ylmethyl)amide

HRID 1432969

반응식

HRID 1432969 반응식
[Cl:1][C:2]1[S:6][C:5]([CH2:7]C(O)=O)=[CH:4][CH:3]=1.CC[N:13]([CH2:16]C)CC.C1(P(N=[N+]=[N-])(C2C=CC=CC=2)=[O:25])C=CC=CC=1.[NH2:35][C:36]1[C:45]2[C:40](=[CH:41][C:42]([CH2:46][N:47]3[CH2:52][CH2:51][NH:50][CH2:49][C:48]3=[O:53])=[CH:43][CH:44]=2)[N:39]=[CH:38][N:37]=1>C(Cl)Cl>[Cl:1][C:2]1[S:6][C:5]([CH2:7][NH:13][C:16]([N:50]2[CH2:51][CH2:52][N:47]([CH2:46][C:42]3[CH:41]=[C:40]4[C:45]([C:36]([NH2:35])=[N:37][CH:38]=[N:39]4)=[CH:44][CH:43]=3)[C:48](=[O:53])[CH2:49]2)=[O:25])=[CH:4][CH:3]=1

참가 물질

6

반응물 4

HCID 8471Triethylamine2회HCID 199401P,P-Diphenylphosphinic azideHCID 44267162-(5-chlorothiophen-2-yl)acetic AcidHCID 179851741-(4-Aminoquinazoline-7-ylmethyl)piperazine-2-one

생성물 1

HCID 540227384-(4-Aminoquinazolin-7-ylmethyl)-3-oxopiperazine-1-carboxylic acid (5-chloro-thiophen-2-ylmethyl)amide수율 4.9%

시약·촉매·용매 1

HCID 6344Dichloromethane용매

실험 절차

To a solution of (5-chloro-thiophen-2-yl)-acetic acid (0.18 g, 1.04 mmol), prepared as described in EXAMPLE 27 in 6 mL of dry CH2Cl2 is added Et3N (0.15 mL g, 1.04 mmol) and diphenylphosphoryl azide (0.24 mL, 1.04 mmol). The mixture is stirred at room temperature for 2.5 h, then heated at 50° C. for 2 hours. To the solution is added 1-(4-aminoquinazoline-7-ylmethyl)piperazine-2-one (0.10 g, 0.41 mmol), EXAMPLE 72, and Et3N (0.15 mL g, 1.04 mmol) and the mixture is heated at 50° C. for 2 h, then stirred at room temperature for 16 hours. The resulting mixture is concentrated. The crude product is purified by RP-HPLC eluting in a gradient of 10% CH3CN/H2O (0.1% TFA) to 60% CH3CN/H2O (0.1% TFA) and the appropriate product fractions are combined and lyopholized to provide the title compound (10 mg, 0.02 mmol) as a white solid. 1H NMR (d6-DMSO, 300 MHz) δ9.69 (bs, 2H), 8.80 (s, 1H), 8.48 (d, 1H), 7.61 (d, 1H), 7.60 (s, 1H), 7.41 (t, 1H), 6.90 (d, 1H), 6.80 (d, 1H), 4.77 (d, 2H), 4.30 (d, 2H), 4.10 (s, 2H), 3.61 (m, 2H), 3.38 (m, 2H). ESI MS, [M+H]+=431,433 (Cl pattern).

후처리

  1. temperatureheated at 50° C. for 2 hours
  2. temperaturethe mixture is heated at 50° C. for 2 h
  3. stirringstirred at room temperature for 16 hours
  4. concentrationThe resulting mixture is concentrated
  5. customThe crude product is purified by RP-HPLC
  6. washeluting in a gradient of 10% CH3CN/H2O (0.1% TFA) to 60% CH3CN/H2O (0.1% TFA)

출처 및 근거

ORD 기록
ord-34fb2b80c4d842bba5c1ea6e0f716829
출처 데이터셋
uspto-grants-2009_11
특허 번호
US07612075B2

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