Sucrose → (2S,3'R,3aR,4R,4'S,5'R,6R,6'R,7S,7aS)-4-[(1S,2R,3S,5R,6S)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1R)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol

HRID 14365

반응식

HRID 14365 반응식
C(O)[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O.[CH3:24][NH:25][C@@H:26]1[C@@H:31]([OH:32])[C@H:30]([O:33][C@@H:34]2[O:39][C@H:38]([CH2:40][OH:41])[C@H:37]([OH:42])[C@@H:36]3[O:43][C:44]4([O:50][C@H:49]([C@@H:51]([NH2:54])[CH2:52][OH:53])[C@H:48]([OH:55])[C@@H:47]([OH:56])[C@H:46]4[OH:57])[O:45][C@H:35]23)[C@@H:29]([OH:58])[C@H:28]([NH2:59])[CH2:27]1>>[CH3:24][NH:25][C@H:26]1[C@H:31]([OH:32])[C@@H:30]([O:33][C@H:34]2[O:39][C@H:38]([CH2:40][OH:41])[C@H:37]([OH:42])[C@@H:36]3[O:43][C@:44]4([O:50][C@H:49]([C@H:51]([NH2:54])[CH2:52][OH:53])[C@H:48]([OH:55])[C@H:47]([OH:56])[C@H:46]4[OH:57])[O:45][C@@H:35]23)[C@H:29]([OH:58])[C@@H:28]([NH2:59])[CH2:27]1

참가 물질

3

반응물 2

HCID 5988SucroseHCID 35766Hygrovetine

생성물 1

HCID 52949613(2S,3'R,3aR,4R,4'S,5'R,6R,6'R,7S,7aS)-4-[(1S,2R,3S,5R,6S)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1R)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol

실험 절차

After completion of the electroshock, 1 ml of Vogel's minimal medium that contained 1.2% sucrose was added to the resultant, and the obtained mixture was then incubated at 30° C. for 2 hours. 200 μl of the obtained solution was applied to and expanded on an agar medium that contained hygromycin B (500 μg/ml). A hygromycin-resistance colony was isolated, and it was then confirmed by PCR whether or not substitution took place in the target locus. In addition, it was also confirmed by the Southern blot method whether or not it contained redundant Hygr gene copies.

후처리

  1. additionwas added to the resultant

출처 및 근거

ORD 기록
ord-0abf829f876f400f8a0f7cb628a03d6a
출처 데이터셋
uspto-grants-2010_06
특허 번호
US07732208B2

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