Potassium Hydroxide → Morphine

HRID 1458505

반응식

HRID 1458505 반응식
Cl.[CH:2]1[C:18]2[CH2:17][C@H:16]3[N:19]([CH2:21][CH2:22][C@@:8]45[C@H:15]3[CH:14]=[CH:13][C@H:11]([OH:12])[C@@H:9]4[O:10][C:6]([C:7]=25)=[C:4]([OH:5])[CH:3]=1)[CH3:20].[OH-].[K+].C1C(CN2C(=O)C=CC2=O)CCC(C(ON2C(=O)CCC2=O)=O)C1>CN(C)C=O>[CH:2]1[C:18]2[CH2:17][C@H:16]3[N:19]([CH2:21][CH2:22][C@@:8]45[C@H:15]3[CH:14]=[CH:13][C@H:11]([OH:12])[C@@H:9]4[O:10][C:6]([C:7]=25)=[C:4]([OH:5])[CH:3]=1)[CH3:20]

참가 물질

5

반응물 3

HCID 14797Potassium HydroxideHCID 125175Succinimidyl 4-(N-maleimidomethyl)cyclohexanecarboxylateHCID 5464110Morphine Hydrochloride

생성물 1

HCID 5288826Morphine

시약·촉매·용매 1

HCID 6228Dimethylformamide용매

실험 절차

Morphine-HCTL (0.068 g, 1.3×10-4 mol, example 9) was dissolved in 5.7 ml of 70% dimethylformamide/30% water and 1.43 ml 1N potassium hydroxide was added. After 5 min the thiol concentration was determined by reaction with DTNB to be 16.9 mM. The morphine thiol -- (6.4 ml, 1.1×10-4 mol) was added with stirring to 26.7 ml SMCC-BSA, 15/1, (0.16 g, 2.4×10-6 mol). The container was purged with argon and the solution was stirred at room temperature for 2 hr. The protein solution was then dialyzed using a PYROSART module against 10 mM (2-(N-morpholino) ethane sulfonic acid, pH 5.77, and 29.5 ml of 5.39 mg/ml BSA-morphine was collected. The protein solution was frozen at -70° C.

후처리

  1. concentrationAfter 5 min the thiol concentration
  2. customwas determined by reaction with DTNB
  3. customThe container was purged with argon
  4. customThe protein solution was then dialyzed
  5. customwas collected
  6. temperatureThe protein solution was frozen at -70° C.

출처 및 근거

ORD 기록
ord-a35981d8995646fbb08b45f0161ebb34
출처 데이터셋
uspto-grants-1998_12
특허 번호
US05851776

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