10-deacetylbaccatin III → 7-xylosyl-baccatin III

HRID 1502227

반응식

HRID 1502227 반응식
[CH3:1][C:2]1[C@@H:19]([OH:20])[CH2:18][C@:14]2([OH:21])[C:15]([CH3:17])([CH3:16])[C:3]=1[C@@H:4]([OH:48])[C:5]([C@@:7]1([CH3:47])[C@H:12]([C@@H:13]2[O:22][C:23]([C:25]2[CH:30]=[CH:29][CH:28]=[CH:27][CH:26]=2)=[O:24])[C@:11]2([O:33][C:34]([CH3:36])=[O:35])[CH2:31][O:32][C@@H:10]2[CH2:9][C@@H:8]1[O:37][C@@H:38]1[O:43][CH2:42][C@@H:41]([OH:44])[C@H:40]([OH:45])[C@H:39]1[OH:46])=[O:6].CC1[C@@H](O)C[C@]2(O)C(C)(C)C=1[C@@H:52](O)[C:53]([C@@]1(C)[C@H]([C@@H]2OC(C2C=CC=CC=2)=O)[C@]2(OC(C)=O)CO[C@@H]2C[C@@H]1O)=[O:54]>>[CH3:1][C:2]1[C@@H:19]([OH:20])[CH2:18][C@:14]2([OH:21])[C:15]([CH3:16])([CH3:17])[C:3]=1[C@@H:4]([O:48][C:53]([CH3:52])=[O:54])[C:5]([C@@:7]1([CH3:47])[C@H:12]([C@@H:13]2[O:22][C:23]([C:25]2[CH:26]=[CH:27][CH:28]=[CH:29][CH:30]=2)=[O:24])[C@:11]2([O:33][C:34]([CH3:36])=[O:35])[CH2:31][O:32][C@@H:10]2[CH2:9][C@@H:8]1[O:37][C@@H:38]1[O:43][CH2:42][C@@H:41]([OH:44])[C@H:40]([OH:45])[C@H:39]1[OH:46])=[O:6]

참가 물질

3

반응물 2

HCID 15427210-deacetylbaccatin IIIHCID 53153567-Xylosyl-10-deacetylbaccatin III2회

생성물 1

HCID 116721247-xylosyl-baccatin III

실험 절차

The strain was the same as that in Example 6 and the substrate was 7-xylosyl-10-deacetylbaccatin III (XDB). 1.5 ml cell reaction solution contained 16 mg dry cells per milliliter and 8 mg XDB per milliliter. The mixture was incubated in a water shaking bath for 24 h at 30˜55° C. HPLC analysis results showed that the conversion ratio of XDB was 86.54% and the yield of the product, 10-deacetylbaccatin III (DB), was 5.57 mg/ml (FIG. 7).

후처리

  1. custom1.5 ml cell reaction solution
  2. dry with materialdry cells per milliliter and 8 mg XDB per milliliter
  3. customHPLC analysis results

출처 및 근거

ORD 기록
ord-06a733c8319e4d919be345dca356d8ca
출처 데이터셋
uspto-grants-2015_12
특허 번호
US09206405B2

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