HCID 15932585, 화합물 → HCID 131717987, 화합물

HRID 1588117노트

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HRID 1588117 반응식
[C:1]([NH:4][C@@H:5]1[C@@H:10]([OH:11])[C@H:9]([OH:12])[C@@H:8]([CH2:13][OH:14])[O:7][C@H:6]1[O:15][C@H:16]1[C@@H:31]([O:32][C@@H:33]2[O:40][C@H:39]([CH2:41][OH:42])[C@@H:37]([OH:38])[C@H:35]([OH:36])[C@H:34]2[NH:43][C:44](=[O:46])[CH3:45])[C@@H:30]([CH2:47][OH:48])[O:29][C@@H:18]([O:19][CH2:20][CH2:21][CH2:22][CH2:23][CH2:24][C:25]([O:27][CH3:28])=[O:26])[C@@H:17]1[OH:49])(=[O:3])[CH3:2].C1C(=O)NC(=O)NC=1[C@@H]1O[C@H](COP(OP(O[C@H:73]2[O:78][C@H:77]([CH2:79][OH:80])[C@H:76]([OH:81])[C@H:75]([OH:82])[C@H:74]2[OH:83])(O)=O)(O)=O)[C@@H](O)[C@H]1O>[As](C)(C)(=O)[O-].[Na+].[Cl-].[Mn+2].[Cl-]>[C@@H:18]1([O:12][C@@H:9]2[C@@H:8]([CH2:13][OH:14])[O:7][C@@H:6]([O:15][C@H:16]3[C@@H:31]([O:32][C@@H:33]4[O:40][C@H:39]([CH2:41][OH:42])[C@@H:37]([O:38][C@@H:73]5[O:78][C@H:77]([CH2:79][OH:80])[C@H:76]([OH:81])[C@H:75]([OH:82])[C@H:74]5[OH:83])[C@H:35]([OH:36])[C@H:34]4[NH:43][C:44](=[O:46])[CH3:45])[C@@H:30]([CH2:47][OH:48])[O:29][C@@H:18]([O:19][CH2:20][CH2:21][CH2:22][CH2:23][CH2:24][C:25]([O:27][CH3:28])=[O:26])[C@@H:17]3[OH:49])[C@H:5]([NH:4][C:1](=[O:3])[CH3:2])[C@H:10]2[OH:11])[O:29][C@H:30]([CH2:47][OH:48])[C@H:31]([OH:32])[C@H:16]([OH:15])[C@H:17]1[OH:49]

참가 물질

5

반응물 2

HCID 15932585HCID 15932585, 화합물HCID 54584734HCID 54584734, 화합물

생성물 1

HCID 131717987HCID 131717987, 화합물

시약·촉매·용매 2

HCID 10313134Manganese(2+) dichloride촉매HCID 2724247Sodium Cacodylate용매

실험 절차

A solution of compound 18 of Example 18 (60 mg), UDP-galactose, (145 mg), bovine galactosyltransferase (6.25 U) and bovine serum albumin (3 mg) in 30 mM sodium cacodylate buffer (5.25 mL, pH 7.0) containing manganese chloride (40 umol) was incubated at 37° C. for 22 h. The reaction mixture was diluted to 20 mL and purified as described in Example 21. Yield of the product was 47 mg. The structural identity of titled compound 24 was unanbiguously assigned by 1H- and 13C-n.m.r (see Tables 4 and 5, respectively).

후처리

  1. additionThe reaction mixture was diluted to 20 mL
  2. custompurified

출처 및 근거

ORD 기록
ord-dc1755a4ffe84d0cad7055dad489b85c
출처 데이터셋
uspto-grants-1993_06
특허 번호
US05220008

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