HCID 15932587, 화합물 → HCID 54227580, 화합물

HRID 1588119노트

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HRID 1588119 반응식
[C:1]([NH:4][C@@H:5]1[C@@H:10]([OH:11])[C@H:9]([OH:12])[C@@H:8]([CH2:13][OH:14])[O:7][C@H:6]1[O:15][C@H:16]1[C@@H:31]([CH2:32][O:33][C@@H:34]2[O:41][C@H:40]([CH2:42][OH:43])[C@@H:38]([OH:39])[C@H:36]([OH:37])[C@H:35]2[NH:44][C:45](=[O:47])[CH3:46])[O:30][C@@H:19]([O:20][CH2:21][CH2:22][CH2:23][CH2:24][CH2:25][C:26]([O:28][CH3:29])=[O:27])[C@H:18]([OH:48])[C@H:17]1[OH:49])(=[O:3])[CH3:2].C1C(=O)NC(=O)NC=1[C@@H]1O[C@H](COP(OP(O[C@H:73]2[O:78][C@H:77]([CH2:79][OH:80])[C@H:76]([OH:81])[C@H:75]([OH:82])[C@H:74]2[OH:83])(O)=O)(O)=O)[C@@H](O)[C@H]1O>[As](C)(C)(=O)[O-].[Na+].[Cl-].[Mn+2].[Cl-]>[C@@H:19]1([O:12][C@@H:9]2[C@@H:8]([CH2:13][OH:14])[O:7][C@@H:6]([O:15][C@H:16]3[C@@H:31]([CH2:32][O:33][C@@H:34]4[O:41][C@H:40]([CH2:42][OH:43])[C@@H:38]([O:39][C@@H:73]5[O:78][C@H:77]([CH2:79][OH:80])[C@H:76]([OH:81])[C@H:75]([OH:82])[C@H:74]5[OH:83])[C@H:36]([OH:37])[C@H:35]4[NH:44][C:45](=[O:47])[CH3:46])[O:30][C@@H:19]([O:20][CH2:21][CH2:22][CH2:23][CH2:24][CH2:25][C:26]([O:28][CH3:29])=[O:27])[C@H:18]([OH:48])[C@H:17]3[OH:49])[C@H:5]([NH:4][C:1](=[O:3])[CH3:2])[C@H:10]2[OH:11])[O:30][C@H:31]([CH2:32][OH:33])[C@H:16]([OH:15])[C@H:17]([OH:49])[C@H:18]1[OH:48]

참가 물질

5

반응물 2

HCID 15932587HCID 15932587, 화합물HCID 54584734HCID 54584734, 화합물

생성물 1

HCID 54227580HCID 54227580, 화합물

시약·촉매·용매 2

HCID 10313134Manganese(2+) dichloride촉매HCID 2724247Sodium Cacodylate용매

실험 절차

A solution of compound 20 of Example 20 (72 mg), UDP-galactose, (190 mg), bovine galactosyltransferase (6.25 U) and bovine serum albumin (3 mg) in 30 mM sodium cacodylate buffer (7 mL, pH 7.0) containing manganese chloride (40 umol) was incubated at 37° C. for 22 h. The reaction mixture was diluted to 20 mL and purified as described in Example 21. Yield of the product was 98 mg. The structural identity of titled compound 26 was unambiguously assigned by 1H- and 13C-n.m.r (see Tables 4 and 5, respectively).

후처리

  1. additionThe reaction mixture was diluted to 20 mL
  2. custompurified

출처 및 근거

ORD 기록
ord-67b967228b3047fcaa75d7d33233d390
출처 데이터셋
uspto-grants-1993_06
특허 번호
US05220008

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