L-(+)-Cysteine → HCID 86604979, 화합물

HRID 1593363

반응식

HRID 1593363 반응식
O.O.O.O.O.O.[Cl-].[Cr+3:8].[Cl-].[Cl-].[NH2:11][C@H:12]([C:15]([OH:17])=[O:16])[CH2:13][SH:14].[P:18]([O:30][CH2:31][C@H:32]1[O:36][C@@H:35]([N:37]2[C:46]3[N:45]=[CH:44][N:43]=[C:41]([NH2:42])[C:40]=3[N:39]=[CH:38]2)[C@H:34]([OH:47])[C@@H:33]1[OH:48])([O:21][P:22]([O:25][P:26]([OH:29])([OH:28])=[O:27])([OH:24])=[O:23])(=[O:20])[OH:19]>O>[NH2:11][C@H:12]([C:15]([OH:17])=[O:16])[CH2:13][SH:14].[Cr:8].[P:18]([O:30][CH2:31][C@H:32]1[O:36][C@@H:35]([N:37]2[C:46]3[N:45]=[CH:44][N:43]=[C:41]([NH2:42])[C:40]=3[N:39]=[CH:38]2)[C@H:34]([OH:47])[C@@H:33]1[OH:48])([O:21][P:22]([O:25][P:26]([OH:28])([OH:29])=[O:27])([OH:24])=[O:23])(=[O:19])[OH:20]

참가 물질

5

반응물 3

HCID 5862L-(+)-CysteineHCID 59575'-AtpHCID 166918Chromium(3+) trichloride hexahydrate

생성물 1

HCID 86604979HCID 86604979, 화합물

시약·촉매·용매 1

HCID 962Water용매

실험 절차

Equimolar amounts of chromium chloride hexahydrate, cysteine, and ATP (10 mM each) were mixed in 50 ml of water at a pH of around 3. The mixture was then incubated at room temperature for 1-2 hours to form the chromium-cysteine-ATP complex. This complex was purified by loading 50 ml of the 10 mM chromium-cysteine-ATP mixture onto a SE53 ion-exchange column having a bed volume of approximately 30 ml. The loaded column was washed with 100 ml of water in order to wash off unbound materials. The bound chromium complex was then eluted with 0.25 M ammonium hydroxide. The eluted fractions (4 ml/fraction) were monitored with a UV spectrophotometer for absorption at wavelengths A260 and A280. An example of the eluting profile is shown in FIG. 1.

후처리

  1. customto form the chromium-cysteine-ATP complex
  2. customThis complex was purified
  3. washThe loaded column was washed with 100 ml of water in order
  4. washto wash off unbound materials
  5. washThe bound chromium complex was then eluted with 0.25 M ammonium hydroxide
  6. customThe eluted fractions (4 ml/fraction) were monitored with a UV spectrophotometer for absorption at wavelengths

출처 및 근거

ORD 기록
ord-067b413668c647c7b20800f34fc9febf
출처 데이터셋
uspto-grants-2007_11
특허 번호
US07297686B2

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