Huagongshe화공사 화학 정보 인쇄 보고서 · 연구 참고용
Sodium Chloride → 2-Isobutyl-3-ketoglutarate diethyl ester
참여 물질
| 역할 | 이름 | HCID | 투입량 |
|---|---|---|---|
| 반응물 | Sodium Chloride | HCID 5234 | — |
| 반응물 | Potassium Phosphate, Tribasic | HCID 62657 | — |
| 반응물 | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| 반응물 | 2-Isobutyl-3-ketoketoglutarate diethyl ester | HCID 22022910 | — |
| 용매 | Dimethyl Sulfoxide | HCID 679 | — |
| 용매 | Glycerol | HCID 753 | — |
| 용매 | Ethyl Acetate | HCID 8857 | — |
| 생성물 | 2-Isobutyl-3-ketoglutarate diethyl ester | HCID 9992659 | — |
반응 단계
To 40 μL of 300 mM potassium phosphate buffer, pH=6.5, containing NaCl (100 mM), DMSO (3% v:v), glucose (200 mM), and glycerol (10% v:v), 40 mM of 2-isobutyl-3-ketoketoglutarate diethyl ester was added along with 100 mg of lyophilized KRED 1001 and 30 mg of glucose dehydrogenase. The reaction mixture was incubated for 48 hours at 37° C. Gas chromatographic analysis showed that the yield of hydroxy diester product was greater than 80%. The product was isolated by extraction of the reaction mixture with ethyl acetate and purification using silica gel chromatography. Isolated yield was in the range of 85–95%.
출처 및 근거
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 특허 번호 | US07301031B2 |
| 출처 데이터셋 | uspto-grants-2007_11 |
| ORD ID | ord-0ef17882d33c428eb99d0d14024b2fc0 |
| 출처 메모 | uspto-grants-2007_11 |