(+)-Lactose → D-Galactose

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Lactose was contacted with UDP-N-acetylglucosamine and a β-galactoside β1-3 N-acetylglucosaminyl transferase purified from pig serum in a 0.1M HEPS buffered aqueous solution at 37° C. The product tetrasaccharide was then contacted with UDP--Gal and a β-N-acetylglucosaminoside β1-4 Galactosyltransferase isolated from bovine milk, in a 0.1M HEPS buffered aqueous solution at 37° C. The product tetrasaccharide was then contacted with CMP--NAN and a β-galactoside α2-6 sialyltransferase isolated from rat liver in a 0.1M HEPES buffered aqueous solution at 37° C. The title pentasaccharide was isolated by conventional methods.

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