Calcium Carbonate → L-Lysine

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A culture medium containing 36 mg/ml glucose, 20 mg/ml ammonium chloride, 1 mg/ml KH2PO4, 0.4 mg/ml MgSO4.7H2O, 10 μg/ml FeSO4.7H2O, 8 μg/ml MnSO4.7H2O, 1 μg/ml (as N) of acid hydrolyzate of soybean protein, 0.1 μg/ml thiamin hydrochloride and 0.3 μg/ml biotin was prepared. Thirty milliliters of this medium was dispensed in 500-ml shake flasks, sterilized by heating at 115° C. for ten minutes, and 1 g each of calcium carbonate, previously sterilized by dry heating, was further added. Each strain listed in Table 2 was innoculated to this medium, and fermentation was continued at 31.5° C. for 48 hours on a reciprocating shaker. Table 3 shows the amount of L-lysine accumulated in each fermentation liquor. As is apparent from the table, all of the drug-resistant strains tested produced L-lysine in good yields.

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